Abstract
Spleen is a secondary lymphoid organ which filters antigens circulating in blood. The importance of spleen as an immune tissue is highlighted by its removal which leads to an increased risk of overwhelming postsplenectomy infection (OPSI). For this reason, spleen auto-transplants are commonly performed to preserve splenic tissue, owing to the remarkable capacity for spleen to regenerate.
In previous experimental models, spleen from day 15 mouse embryos were shown to regenerate following transplantation under the kidney capsule (Glanville, 2009). In this study, murine spleen tissue was shown to regenerate from neonatal spleen capsule alone. Spleen capsules isolated from 3 day-old mice and transplanted under the kidney capsule of adult recipients regenerated splenic tissue after 4 weeks, displaying structures including red and white pulp, T and B cell compartments, follicular dendritic cell networks, marginal zones and conduit structures. Regenerated spleens also demonstrated functional capacity supporting antibody class switching and affinity maturation.
Transplantation efficiency was dependent on donor age with capsules isolated from 3 day-old mice more successful than 8 day-old ¬¬spleen capsules. In contrast, recipient age did not appear to be important with transplantations equally successful from both 6 and 30 week-old recipients. We are now attempting to identify stromal cells in spleen capsule that initiate spleen neogenesis. Overall, these results are expected to lead towards development of artificial spleen.
In previous experimental models, spleen from day 15 mouse embryos were shown to regenerate following transplantation under the kidney capsule (Glanville, 2009). In this study, murine spleen tissue was shown to regenerate from neonatal spleen capsule alone. Spleen capsules isolated from 3 day-old mice and transplanted under the kidney capsule of adult recipients regenerated splenic tissue after 4 weeks, displaying structures including red and white pulp, T and B cell compartments, follicular dendritic cell networks, marginal zones and conduit structures. Regenerated spleens also demonstrated functional capacity supporting antibody class switching and affinity maturation.
Transplantation efficiency was dependent on donor age with capsules isolated from 3 day-old mice more successful than 8 day-old ¬¬spleen capsules. In contrast, recipient age did not appear to be important with transplantations equally successful from both 6 and 30 week-old recipients. We are now attempting to identify stromal cells in spleen capsule that initiate spleen neogenesis. Overall, these results are expected to lead towards development of artificial spleen.
| Original language | English |
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| Publication status | Published - 17 Dec 2010 |
| Externally published | Yes |
| Event | The 2nd Workshop of Synthetic Immunology - Kyoto University, Kyoto, Japan Duration: 17 Dec 2010 → 18 Dec 2010 |
Workshop
| Workshop | The 2nd Workshop of Synthetic Immunology |
|---|---|
| Country/Territory | Japan |
| City | Kyoto |
| Period | 17/12/10 → 18/12/10 |