Monosaccharide inhibition of cytotoxic T-cell function: demonstration of clone-specific effects

H C O'Neill, C R Parish

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

A range of monosaccharides has been tested for their capacity to influence the induction and effector function of alloreactive cytotoxic T (Tc) cells. Strain-specific differences in the capacity of monosaccharides to inhibit Tc cell induction have been demonstrated. Monosaccharides can also inhibit effector function of target cell lysis, but this could only be demonstrated by assessing the effect of sugars added to limiting dilution cultures of alloantigen-stimulated T cells. B10.A(4R) anti-BALB/c Tc cells have been reproducibly inhibited by D-glucosamine and D-galactosamine, as well as D-galacturonic acid, at both the induction and effector phases of the Tc cell response. Analysis of monosaccharide inhibition of cytotoxicity in limiting dilution cultures has confirmed that D-glucosamine is the most effective inhibitor of B10.A(4R) anti-BALB/c Tc cells, while D-galactosamine and D-galacturonic acid inhibit cytotoxicity in only some limiting dilution wells. Analysis of several B10.A(4R) anti-BALB/c Tc cell clones has revealed at least two different 'clone-specific' patterns of inhibition by D-glucose, D-glucuronic acid and D-galacturonic acid. Since Tc cell recognition of antigen is generally specific for class I major histocompatibility complex (MHC) antigens, this data implicates a role for MHC-associated carbohydrate structures expressed by target cells in T-lymphocyte interactions with antigen.

Original languageEnglish
Pages (from-to)181-184
Number of pages4
JournalImmunology
Volume64
Issue number1
Publication statusPublished - May 1988
Externally publishedYes

Fingerprint

Monosaccharides
Clone Cells
T-Lymphocytes
Galactosamine
Glucosamine
Major Histocompatibility Complex
Antigens
Glucuronic Acid
Histocompatibility Antigens Class I
Isoantigens
Carbohydrates
Glucose

Cite this

@article{5f5a4b19877b44b78fe59f5d0cc785f7,
title = "Monosaccharide inhibition of cytotoxic T-cell function: demonstration of clone-specific effects",
abstract = "A range of monosaccharides has been tested for their capacity to influence the induction and effector function of alloreactive cytotoxic T (Tc) cells. Strain-specific differences in the capacity of monosaccharides to inhibit Tc cell induction have been demonstrated. Monosaccharides can also inhibit effector function of target cell lysis, but this could only be demonstrated by assessing the effect of sugars added to limiting dilution cultures of alloantigen-stimulated T cells. B10.A(4R) anti-BALB/c Tc cells have been reproducibly inhibited by D-glucosamine and D-galactosamine, as well as D-galacturonic acid, at both the induction and effector phases of the Tc cell response. Analysis of monosaccharide inhibition of cytotoxicity in limiting dilution cultures has confirmed that D-glucosamine is the most effective inhibitor of B10.A(4R) anti-BALB/c Tc cells, while D-galactosamine and D-galacturonic acid inhibit cytotoxicity in only some limiting dilution wells. Analysis of several B10.A(4R) anti-BALB/c Tc cell clones has revealed at least two different 'clone-specific' patterns of inhibition by D-glucose, D-glucuronic acid and D-galacturonic acid. Since Tc cell recognition of antigen is generally specific for class I major histocompatibility complex (MHC) antigens, this data implicates a role for MHC-associated carbohydrate structures expressed by target cells in T-lymphocyte interactions with antigen.",
author = "O'Neill, {H C} and Parish, {C R}",
year = "1988",
month = "5",
language = "English",
volume = "64",
pages = "181--184",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "1",

}

Monosaccharide inhibition of cytotoxic T-cell function : demonstration of clone-specific effects. / O'Neill, H C; Parish, C R.

In: Immunology, Vol. 64, No. 1, 05.1988, p. 181-184.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Monosaccharide inhibition of cytotoxic T-cell function

T2 - demonstration of clone-specific effects

AU - O'Neill, H C

AU - Parish, C R

PY - 1988/5

Y1 - 1988/5

N2 - A range of monosaccharides has been tested for their capacity to influence the induction and effector function of alloreactive cytotoxic T (Tc) cells. Strain-specific differences in the capacity of monosaccharides to inhibit Tc cell induction have been demonstrated. Monosaccharides can also inhibit effector function of target cell lysis, but this could only be demonstrated by assessing the effect of sugars added to limiting dilution cultures of alloantigen-stimulated T cells. B10.A(4R) anti-BALB/c Tc cells have been reproducibly inhibited by D-glucosamine and D-galactosamine, as well as D-galacturonic acid, at both the induction and effector phases of the Tc cell response. Analysis of monosaccharide inhibition of cytotoxicity in limiting dilution cultures has confirmed that D-glucosamine is the most effective inhibitor of B10.A(4R) anti-BALB/c Tc cells, while D-galactosamine and D-galacturonic acid inhibit cytotoxicity in only some limiting dilution wells. Analysis of several B10.A(4R) anti-BALB/c Tc cell clones has revealed at least two different 'clone-specific' patterns of inhibition by D-glucose, D-glucuronic acid and D-galacturonic acid. Since Tc cell recognition of antigen is generally specific for class I major histocompatibility complex (MHC) antigens, this data implicates a role for MHC-associated carbohydrate structures expressed by target cells in T-lymphocyte interactions with antigen.

AB - A range of monosaccharides has been tested for their capacity to influence the induction and effector function of alloreactive cytotoxic T (Tc) cells. Strain-specific differences in the capacity of monosaccharides to inhibit Tc cell induction have been demonstrated. Monosaccharides can also inhibit effector function of target cell lysis, but this could only be demonstrated by assessing the effect of sugars added to limiting dilution cultures of alloantigen-stimulated T cells. B10.A(4R) anti-BALB/c Tc cells have been reproducibly inhibited by D-glucosamine and D-galactosamine, as well as D-galacturonic acid, at both the induction and effector phases of the Tc cell response. Analysis of monosaccharide inhibition of cytotoxicity in limiting dilution cultures has confirmed that D-glucosamine is the most effective inhibitor of B10.A(4R) anti-BALB/c Tc cells, while D-galactosamine and D-galacturonic acid inhibit cytotoxicity in only some limiting dilution wells. Analysis of several B10.A(4R) anti-BALB/c Tc cell clones has revealed at least two different 'clone-specific' patterns of inhibition by D-glucose, D-glucuronic acid and D-galacturonic acid. Since Tc cell recognition of antigen is generally specific for class I major histocompatibility complex (MHC) antigens, this data implicates a role for MHC-associated carbohydrate structures expressed by target cells in T-lymphocyte interactions with antigen.

M3 - Article

VL - 64

SP - 181

EP - 184

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 1

ER -