Monoclonal antibodies which identify carbohydrate-defined MHC class I epitopes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Eleven different monoclonal antibodies specific for H-2K- and H-2D-encoded Class I molecules have been screened to determine Class I epitopes dependent on both carbohydrate and protein structures. Monoclonal antibodies have been identified which bind to carbohydrate-defined antigens encoded by both the H-2K and H-2D gene regions. Sensitivity to glycosidases versus pronase has been used to classify antigens both expressed as cell surface molecules and when prepared as detergent solubilized antigen. Several simple sugars have also been found to act as inhibitors of antibodies which bind to carbohydrate-defined sites. The genetic control of carbohydrate antigen expression by H-2K- and H-2D-linked genes has been verified since a specific antibody does not bind to H-2Kb or H-2Db molecules encoded by several mutant strains of mice containing single amino acid substitutions in their protein product. All of these data are consistent with Class I antigenic structures being encoded in carbohydrate as well as protein moieties.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalImmunology and Cell Biology
Volume69
Issue number3
DOIs
Publication statusPublished - Jun 1991
Externally publishedYes

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Epitopes
Monoclonal Antibodies
Carbohydrates
Antigens
Mutant Strains Mice
Pronase
Proteins
Antibodies
Glycoside Hydrolases
Amino Acid Substitution
Detergents
Genes

Cite this

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title = "Monoclonal antibodies which identify carbohydrate-defined MHC class I epitopes",
abstract = "Eleven different monoclonal antibodies specific for H-2K- and H-2D-encoded Class I molecules have been screened to determine Class I epitopes dependent on both carbohydrate and protein structures. Monoclonal antibodies have been identified which bind to carbohydrate-defined antigens encoded by both the H-2K and H-2D gene regions. Sensitivity to glycosidases versus pronase has been used to classify antigens both expressed as cell surface molecules and when prepared as detergent solubilized antigen. Several simple sugars have also been found to act as inhibitors of antibodies which bind to carbohydrate-defined sites. The genetic control of carbohydrate antigen expression by H-2K- and H-2D-linked genes has been verified since a specific antibody does not bind to H-2Kb or H-2Db molecules encoded by several mutant strains of mice containing single amino acid substitutions in their protein product. All of these data are consistent with Class I antigenic structures being encoded in carbohydrate as well as protein moieties.",
author = "O'Neill, {H C}",
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Monoclonal antibodies which identify carbohydrate-defined MHC class I epitopes. / O'Neill, H C.

In: Immunology and Cell Biology, Vol. 69 , No. 3, 06.1991, p. 159-165.

Research output: Contribution to journalArticleResearchpeer-review

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