Mitochondrial inhibition in rat retinal cell cultures as a model of metabolic compromise: Mechanisms of injury and neuroprotection

John P.M. Wood, Teresa Mammone, Glyn Chidlow, Tim Greenwell, Robert J. Casson

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)


PURPOSE. Our study aimed to establish a model of energetic and metabolic dysfunction to cultured retinal cells by chemically inhibiting the mitochondrial electron transport chain with sodium azide (NaN3), and subsequently investigating toxic mechanisms and potential neuroprotective strategies. 

METHODS. Mixed rat retinal cultures comprising neurons and glia were treated with a range of NaN3 concentrations for up to 24 hours and toxicity levels were determined by immunologic methods. Detailed pathologic mechanisms were investigated by assessing apoptosis (TUNEL assay), mitochondrial membrane potential, reactive oxygen species (ROS), and levels of adenosine triphosphate (ATP). Finally, a number of pharmacologic agents were tested to determine whether they could abrogate the effects of NaN3 to retinal cells. 

RESULTS. Neurons and glia were killed by NaN3 in a concentration- and time-dependent manner, with neurons being relatively more susceptible. Cell loss was via apoptosis for glia but not for neurons. Cell death generally involved a loss of mitochondrial membrane potential, a reduction in cellular ATP, and an increase in intracellular ROS levels. Glucose was partially able to prevent neuron death, as were the antioxidants trolox and pyruvate, calpain inhibitor III, the ryanodine receptor blocker dantrolene, and the nitric oxide synthase inhibitor L-NAME. 

CONCLUSIONS. Mitochondrial respiratory inhibition via NaN3 treatment, with delineated mechanisms of toxicity and neuroprotection, represents a valid and reproducible metabolic challenge to cultured retinal cells.

Original languageEnglish
Pages (from-to)4897-4909
Number of pages13
JournalInvestigative Ophthalmology and Visual Science
Issue number8
Publication statusPublished - Jul 2012
Externally publishedYes


Dive into the research topics of 'Mitochondrial inhibition in rat retinal cell cultures as a model of metabolic compromise: Mechanisms of injury and neuroprotection'. Together they form a unique fingerprint.

Cite this