TY - JOUR
T1 - Macrophage migration inhibitory factor and its binding partner HTRA1 are expressed by olfactory ensheathing cells
AU - Wright, A. A.
AU - Todorovic, M.
AU - Murtaza, M.
AU - St John, J. A.
AU - Ekberg, J. A.
N1 - Funding Information:
This work was supported by a Perry Cross Spinal Research Foundation, Australia, Grant to JE and JSJ, a Clem Jones Foundation, Australia, Grant to JSJ and JE, and an ARC Discovery Grant to JE and JSJ ( DP150104495 ). We thank Dr Anu Chacko and Dr Ali Delbaz for assistance with the Elisa assays.
Publisher Copyright:
© 2019 The Authors
PY - 2020/1
Y1 - 2020/1
N2 - Macrophage migration inhibitory factor (MIF) is an important regulator of innate immunity with key roles in neural regeneration and responses to pathogens, amongst a multitude of other functions. The expression of MIF and its binding partners has been characterised throughout the nervous system, with one key exception: the primary olfactory nervous system. Here, we showed in young mice (postnatal day 10) that MIF is expressed in the olfactory nerve by olfactory ensheathing glial cells (OECs) and by olfactory nerve fibroblasts. We also examined the expression of potential binding partners for MIF, and found that the serine protease HTRA1, known to be inhibited by MIF, was also expressed at high levels by OECs and olfactory fibroblasts in vivo and in vitro. We also demonstrated that MIF mediated segregation between OECs and J774a.1 cells (a monocyte/macrophage cell line) in co-culture, which suggests that MIF contributes to the fact that macrophages are largely absent from olfactory nerve fascicles. Phagocytosis assays of axonal debris demonstrated that MIF strongly stimulates phagocytosis by OECs, which indicates that MIF may play a role in the response of OECs to the continual turnover of olfactory axons that occurs throughout life.
AB - Macrophage migration inhibitory factor (MIF) is an important regulator of innate immunity with key roles in neural regeneration and responses to pathogens, amongst a multitude of other functions. The expression of MIF and its binding partners has been characterised throughout the nervous system, with one key exception: the primary olfactory nervous system. Here, we showed in young mice (postnatal day 10) that MIF is expressed in the olfactory nerve by olfactory ensheathing glial cells (OECs) and by olfactory nerve fibroblasts. We also examined the expression of potential binding partners for MIF, and found that the serine protease HTRA1, known to be inhibited by MIF, was also expressed at high levels by OECs and olfactory fibroblasts in vivo and in vitro. We also demonstrated that MIF mediated segregation between OECs and J774a.1 cells (a monocyte/macrophage cell line) in co-culture, which suggests that MIF contributes to the fact that macrophages are largely absent from olfactory nerve fascicles. Phagocytosis assays of axonal debris demonstrated that MIF strongly stimulates phagocytosis by OECs, which indicates that MIF may play a role in the response of OECs to the continual turnover of olfactory axons that occurs throughout life.
UR - http://www.scopus.com/inward/record.url?scp=85075970684&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2019.103450
DO - 10.1016/j.mcn.2019.103450
M3 - Article
C2 - 31794879
AN - SCOPUS:85075970684
SN - 1044-7431
VL - 102
SP - 1
EP - 14
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
M1 - 103450
ER -