Low muscle glycogen concentration does not suppress the anabolic response to resistance exercise

Donny M. Camera, Daniel W D West, Nicholas A. Burd, Stuart M. Phillips, Andrew P. Garnham, John A. Hawley, Vernon G. Coffey

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

We determined the effect of muscle glycogen concentration and postexercise nutrition on anabolic signaling and rates of myofibrillar protein synthesis after resistance exercise (REX). Sixteen young, healthy men matched for age, body mass, peak oxygen uptake (VO2peak) and strength (one repetition maximum; 1RM) were randomly assigned to either a nutrient or placebo group. After 48 h diet and exercise control, subjects undertook a glycogen-depletion protocol consisting of one-leg cycling to fatigue (LOW), whereas the other leg rested (NORM). The next morning following an overnight fast, a primed, constant infusion of L-[ring-13C6] phenylalanine was commenced and subjects completed 8 sets of 5 unilateral leg press repetitions at 80% 1RM. Immediately after REX and 2 h later, subjects consumed a 500 ml bolus of a protein/CHO (20 g whey + 40 g maltodextrin) or placebo beverage. Muscle biopsies from the vastus lateralis of both legs were taken at rest and 1 and 4 h after REX. Muscle glycogen concentration was higher in the NORM than LOW at all time points in both nutrient and placebo groups (P < 0.05). Postexercise Akt-p70S6K-rpS6 phosphorylation increased in both groups with no differences between legs (P < 0.05). mTORSer2448 phosphorylation in placebo increased 1 h after exercise in NORM (P < 0.05), whereas mTOR increased ∼4-fold in LOW (P < 0.01) and ∼11 fold in NORM with nutrient (P < 0.01; different between legs P < 0.05). Post-exercise rates of MPS were not different between NORM and LOW in nutrient (0.070 ± 0.022 vs. 0.068 ± 0.018 %/h) or placebo (0.045 ± 0.021 vs. 0.049 ± 0.017 %/h). We conclude that commencing high-intensity REX with low muscle glycogen availability does not compromise the anabolic signal and subsequent rates of MPS, at least during the early (4 h) postexercise recovery period.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalJournal of Applied Physiology
Volume113
Issue number2
DOIs
Publication statusPublished - 15 Jul 2012
Externally publishedYes

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Glycogen
Leg
Exercise
Muscles
Placebos
Food
Phosphorylation
70-kDa Ribosomal Protein S6 Kinases
Beverages
Quadriceps Muscle
Phenylalanine
Fatigue
Proteins
Oxygen
Diet
Biopsy

Cite this

Camera, Donny M. ; West, Daniel W D ; Burd, Nicholas A. ; Phillips, Stuart M. ; Garnham, Andrew P. ; Hawley, John A. ; Coffey, Vernon G. / Low muscle glycogen concentration does not suppress the anabolic response to resistance exercise. In: Journal of Applied Physiology. 2012 ; Vol. 113, No. 2. pp. 206-214.
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abstract = "We determined the effect of muscle glycogen concentration and postexercise nutrition on anabolic signaling and rates of myofibrillar protein synthesis after resistance exercise (REX). Sixteen young, healthy men matched for age, body mass, peak oxygen uptake (VO2peak) and strength (one repetition maximum; 1RM) were randomly assigned to either a nutrient or placebo group. After 48 h diet and exercise control, subjects undertook a glycogen-depletion protocol consisting of one-leg cycling to fatigue (LOW), whereas the other leg rested (NORM). The next morning following an overnight fast, a primed, constant infusion of L-[ring-13C6] phenylalanine was commenced and subjects completed 8 sets of 5 unilateral leg press repetitions at 80{\%} 1RM. Immediately after REX and 2 h later, subjects consumed a 500 ml bolus of a protein/CHO (20 g whey + 40 g maltodextrin) or placebo beverage. Muscle biopsies from the vastus lateralis of both legs were taken at rest and 1 and 4 h after REX. Muscle glycogen concentration was higher in the NORM than LOW at all time points in both nutrient and placebo groups (P < 0.05). Postexercise Akt-p70S6K-rpS6 phosphorylation increased in both groups with no differences between legs (P < 0.05). mTORSer2448 phosphorylation in placebo increased 1 h after exercise in NORM (P < 0.05), whereas mTOR increased ∼4-fold in LOW (P < 0.01) and ∼11 fold in NORM with nutrient (P < 0.01; different between legs P < 0.05). Post-exercise rates of MPS were not different between NORM and LOW in nutrient (0.070 ± 0.022 vs. 0.068 ± 0.018 {\%}/h) or placebo (0.045 ± 0.021 vs. 0.049 ± 0.017 {\%}/h). We conclude that commencing high-intensity REX with low muscle glycogen availability does not compromise the anabolic signal and subsequent rates of MPS, at least during the early (4 h) postexercise recovery period.",
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Low muscle glycogen concentration does not suppress the anabolic response to resistance exercise. / Camera, Donny M.; West, Daniel W D; Burd, Nicholas A.; Phillips, Stuart M.; Garnham, Andrew P.; Hawley, John A.; Coffey, Vernon G.

In: Journal of Applied Physiology, Vol. 113, No. 2, 15.07.2012, p. 206-214.

Research output: Contribution to journalArticleResearchpeer-review

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AU - West, Daniel W D

AU - Burd, Nicholas A.

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