Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells

Johana Tello Velasquez, Michelle E. Watts, Michael Todorovic, Lynnmaria Nazareth, Erika Pastrana, Javier Diaz-Nido, Filip Lim, Jenny A K Ekberg, Ronald J. Quinn, James A. St John

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

One of the promising strategies for neural repair therapies is the transplantation of olfactory ensheathing cells (OECs) which are the glial cells of the olfactory system. We evaluated the effects of curcumin on the behaviour of mouse OECs to determine if it could be of use to further enhance the therapeutic potential of OECs. Curcumin, a natural polyphenol compound found in the spice turmeric, is known for its anti-cancer properties at doses over 10 μM, and often at 50 μM, and it exerts its effects on cancer cells in part by activation of MAP kinases. In contrast, we found that low-dose curcumin (0.5 μM) applied to OECs strikingly modulated the dynamic morphology, increased the rate of migration by up to 4-fold, and promoted significant proliferation of the OECs. Most dramatically, low-dose curcumin stimulated a 10-fold increase in the phagocytic activity of OECs. All of these potently stimulated behavioural characteristics of OECs are favourable for neural repair therapies. Importantly, low-dose curcumin gave a transient activation of p38 kinases, which is in contrast to the high dose curcumin effects on cancer cells in which these MAP kinases tend to undergo prolonged activation. Low-dose curcumin mediated effects on OECs demonstrate cell-type specific stimulation of p38 and ERK kinases. These results constitute the first evidence that low-dose curcumin can modulate the behaviour of olfactory glia into a phenotype potentially more favourable for neural repair and thereby improve the therapeutic use of OECs for neural repair therapies.

Original languageEnglish
Article numbere111787
JournalPLoS One
Volume9
Issue number10
DOIs
Publication statusPublished - 31 Oct 2014
Externally publishedYes

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Curcumin
curcumin
phagocytosis
dosage
Repair
Phosphotransferases
cells
therapeutics
mitogen-activated protein kinase
Chemical activation
Cells
phosphotransferases (kinases)
Neuroglia
Polyphenols
turmeric
neuroglia
Curcuma
Neoplasms
Spices
spices

Cite this

Velasquez, J. T., Watts, M. E., Todorovic, M., Nazareth, L., Pastrana, E., Diaz-Nido, J., ... St John, J. A. (2014). Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells. PLoS One, 9(10), [e111787]. https://doi.org/10.1371/journal.pone.0111787
Velasquez, Johana Tello ; Watts, Michelle E. ; Todorovic, Michael ; Nazareth, Lynnmaria ; Pastrana, Erika ; Diaz-Nido, Javier ; Lim, Filip ; Ekberg, Jenny A K ; Quinn, Ronald J. ; St John, James A. / Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells. In: PLoS One. 2014 ; Vol. 9, No. 10.
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abstract = "One of the promising strategies for neural repair therapies is the transplantation of olfactory ensheathing cells (OECs) which are the glial cells of the olfactory system. We evaluated the effects of curcumin on the behaviour of mouse OECs to determine if it could be of use to further enhance the therapeutic potential of OECs. Curcumin, a natural polyphenol compound found in the spice turmeric, is known for its anti-cancer properties at doses over 10 μM, and often at 50 μM, and it exerts its effects on cancer cells in part by activation of MAP kinases. In contrast, we found that low-dose curcumin (0.5 μM) applied to OECs strikingly modulated the dynamic morphology, increased the rate of migration by up to 4-fold, and promoted significant proliferation of the OECs. Most dramatically, low-dose curcumin stimulated a 10-fold increase in the phagocytic activity of OECs. All of these potently stimulated behavioural characteristics of OECs are favourable for neural repair therapies. Importantly, low-dose curcumin gave a transient activation of p38 kinases, which is in contrast to the high dose curcumin effects on cancer cells in which these MAP kinases tend to undergo prolonged activation. Low-dose curcumin mediated effects on OECs demonstrate cell-type specific stimulation of p38 and ERK kinases. These results constitute the first evidence that low-dose curcumin can modulate the behaviour of olfactory glia into a phenotype potentially more favourable for neural repair and thereby improve the therapeutic use of OECs for neural repair therapies.",
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Velasquez, JT, Watts, ME, Todorovic, M, Nazareth, L, Pastrana, E, Diaz-Nido, J, Lim, F, Ekberg, JAK, Quinn, RJ & St John, JA 2014, 'Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells' PLoS One, vol. 9, no. 10, e111787. https://doi.org/10.1371/journal.pone.0111787

Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells. / Velasquez, Johana Tello; Watts, Michelle E.; Todorovic, Michael; Nazareth, Lynnmaria; Pastrana, Erika; Diaz-Nido, Javier; Lim, Filip; Ekberg, Jenny A K; Quinn, Ronald J.; St John, James A.

In: PLoS One, Vol. 9, No. 10, e111787, 31.10.2014.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Velasquez, Johana Tello

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AU - Todorovic, Michael

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AU - Diaz-Nido, Javier

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AB - One of the promising strategies for neural repair therapies is the transplantation of olfactory ensheathing cells (OECs) which are the glial cells of the olfactory system. We evaluated the effects of curcumin on the behaviour of mouse OECs to determine if it could be of use to further enhance the therapeutic potential of OECs. Curcumin, a natural polyphenol compound found in the spice turmeric, is known for its anti-cancer properties at doses over 10 μM, and often at 50 μM, and it exerts its effects on cancer cells in part by activation of MAP kinases. In contrast, we found that low-dose curcumin (0.5 μM) applied to OECs strikingly modulated the dynamic morphology, increased the rate of migration by up to 4-fold, and promoted significant proliferation of the OECs. Most dramatically, low-dose curcumin stimulated a 10-fold increase in the phagocytic activity of OECs. All of these potently stimulated behavioural characteristics of OECs are favourable for neural repair therapies. Importantly, low-dose curcumin gave a transient activation of p38 kinases, which is in contrast to the high dose curcumin effects on cancer cells in which these MAP kinases tend to undergo prolonged activation. Low-dose curcumin mediated effects on OECs demonstrate cell-type specific stimulation of p38 and ERK kinases. These results constitute the first evidence that low-dose curcumin can modulate the behaviour of olfactory glia into a phenotype potentially more favourable for neural repair and thereby improve the therapeutic use of OECs for neural repair therapies.

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Velasquez JT, Watts ME, Todorovic M, Nazareth L, Pastrana E, Diaz-Nido J et al. Low-dose curcumin stimulates proliferation, migration and phagocytic activity of olfactory ensheathing cells. PLoS One. 2014 Oct 31;9(10). e111787. https://doi.org/10.1371/journal.pone.0111787