Long-term stromal cultures produce dendritic-like cells

Keping Ni, Helen C O'Neill

Research output: Contribution to journalArticleResearchpeer-review

50 Citations (Scopus)

Abstract

A stromal cell-dependent long-term culture (LTC) system has been developed from spleen which continuously generates non-adherent cells with dendritic cell (DC) characteristics. Bioassays using factor-dependent cell lines have revealed that both spleen and thymic stromal cultures secrete interleukin (IL)-3 and IL-6-like growth factors. Conditioned medium from LTC also contains factors which appear to be unrelated to IL-3 and induces growth of stromal cells from bone marrow. Non-adherent cells generated in LTC were not T or B lymphoid cells or granulocytes, nor were mast cells detectable. Morphological and electron microscopic examination has also excluded the presence of macrophages (Mo). Cells with DC morphology have been detected by both light and electron microscopy. The majority of cells in the non-adherent population were found to have multiple membrane pseudopodia, with a small acentric nucleus. These appear to be the precursors of DC. They expressed cell surface markers detectable with DC- specific antibodies and antibody specific for major histocompability complex Class II molecules. A proportion of cells also expressed myeloid markers, but since this expression was not supported by histochemical staining for myeloperoxidase or non-specific esterase, it was concluded that the cells produced are not typical of the myeloid lineage. Cells generated in LTC were shown to be potent stimulators for allogeneic and syngeneic MLR and for antigen-specific T-cell proliferation.

Original languageEnglish
Pages (from-to)710-725
Number of pages16
JournalBritish Journal of Haematology
Volume97
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Dendritic Cells
Interleukin-3
Spleen
Carboxylesterase
Pseudopodia
Antibodies
Stromal Cells
Conditioned Culture Medium
Mesenchymal Stromal Cells
Granulocytes
Mast Cells
Biological Assay
Peroxidase
Interleukin-6
Intercellular Signaling Peptides and Proteins
Electron Microscopy
Macrophages
Cell Proliferation
Lymphocytes
Electrons

Cite this

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title = "Long-term stromal cultures produce dendritic-like cells",
abstract = "A stromal cell-dependent long-term culture (LTC) system has been developed from spleen which continuously generates non-adherent cells with dendritic cell (DC) characteristics. Bioassays using factor-dependent cell lines have revealed that both spleen and thymic stromal cultures secrete interleukin (IL)-3 and IL-6-like growth factors. Conditioned medium from LTC also contains factors which appear to be unrelated to IL-3 and induces growth of stromal cells from bone marrow. Non-adherent cells generated in LTC were not T or B lymphoid cells or granulocytes, nor were mast cells detectable. Morphological and electron microscopic examination has also excluded the presence of macrophages (Mo). Cells with DC morphology have been detected by both light and electron microscopy. The majority of cells in the non-adherent population were found to have multiple membrane pseudopodia, with a small acentric nucleus. These appear to be the precursors of DC. They expressed cell surface markers detectable with DC- specific antibodies and antibody specific for major histocompability complex Class II molecules. A proportion of cells also expressed myeloid markers, but since this expression was not supported by histochemical staining for myeloperoxidase or non-specific esterase, it was concluded that the cells produced are not typical of the myeloid lineage. Cells generated in LTC were shown to be potent stimulators for allogeneic and syngeneic MLR and for antigen-specific T-cell proliferation.",
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Long-term stromal cultures produce dendritic-like cells. / Ni, Keping; O'Neill, Helen C.

In: British Journal of Haematology, Vol. 97, No. 4, 1997, p. 710-725.

Research output: Contribution to journalArticleResearchpeer-review

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