Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells

Johana Tello Velasquez, Rebecca Qing Yao, Filip Lim, Chunguang Han, Makoto Ojika, Jenny A K Ekberg, Ronald J. Quinn, James A St John

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Linckosides are members of the steroid glycoside family isolated from the starfish Linckia laevigata. These natural compounds have notable neuritogenic activity and synergistic effects on NGF-induced neuronal differentiation of PC12 cells. Neurogenic factors or molecules that are able to mimic their activities are known to be involved in the survival, proliferation and migration of neurons and glial cells; however how glial cells respond to specific neurogenic molecules such as linckosides has not been investigated. This study aimed to examine the effect of three different linckosides (linckoside A, B and granulatoside A) on the morphological properties, proliferation and migration of human olfactory ensheathing cells (hOECs). The proliferation rate after all the treatments was higher than control as detected by MTS assay. Additionally, hOECs displayed dramatic morphological changes characterized by a higher number of processes after linckoside treatment. Interestingly changes in microtubule organization and expression levels of some early neuronal markers (GAP43 and βIII-tubulin) were also observed. An increase in the phosphorylation of ERK 1/2 after addition of the compounds suggests that this pathway may be involved in the linckoside-mediated effects particularly those related to morphological changes. These results are the first description of the stimulating effects of linckosides on hOECs and raise the potential for this natural compound or its derivatives to be used to regulate and enhance the therapeutic properties of OECs, particularly for cell transplantation therapies.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalMolecular and Cellular Neurosciences
Volume75
DOIs
Publication statusPublished - 1 Sep 2016
Externally publishedYes

Fingerprint

Neuroglia
Starfish
PC12 Cells
Cell Transplantation
Nerve Growth Factor
Tubulin
Glycosides
Cell- and Tissue-Based Therapy
Microtubules
Therapeutics
Steroids
Phosphorylation
Neurons
Survival
linckoside A
linckoside B
granulatoside A

Cite this

Tello Velasquez, J., Yao, R. Q., Lim, F., Han, C., Ojika, M., Ekberg, J. A. K., ... John, J. A. S. (2016). Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells. Molecular and Cellular Neurosciences, 75, 1-13. https://doi.org/10.1016/j.mcn.2016.06.005
Tello Velasquez, Johana ; Yao, Rebecca Qing ; Lim, Filip ; Han, Chunguang ; Ojika, Makoto ; Ekberg, Jenny A K ; Quinn, Ronald J. ; John, James A St. / Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells. In: Molecular and Cellular Neurosciences. 2016 ; Vol. 75. pp. 1-13.
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Tello Velasquez, J, Yao, RQ, Lim, F, Han, C, Ojika, M, Ekberg, JAK, Quinn, RJ & John, JAS 2016, 'Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells' Molecular and Cellular Neurosciences, vol. 75, pp. 1-13. https://doi.org/10.1016/j.mcn.2016.06.005

Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells. / Tello Velasquez, Johana; Yao, Rebecca Qing; Lim, Filip; Han, Chunguang; Ojika, Makoto; Ekberg, Jenny A K; Quinn, Ronald J.; John, James A St.

In: Molecular and Cellular Neurosciences, Vol. 75, 01.09.2016, p. 1-13.

Research output: Contribution to journalArticleResearchpeer-review

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