Isometric thumb exertion induces B cell and T cell lymphocytosis in trained and untrained males: Physical aptitude determines response profiles

Adam Michael Szlezak, Lotti Tajouri, James Keane, Siri Lauleten Lauluten, Clare Minahan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Purpose: The present study examined the effect of low-dose thumb exertion on lymphocyte subpopulation trafficking. The potential role of blood lactate in mediating lymphocyte redistribution was also investigated. Methods: 27 male participants (18 weightlifting-trained; 9 untrained) were separated into 3 groups of 9 (Weightlifting and Untrained Experimental: WLEXP, UTEXP; Weightlifting Placebo: WLPLA). WLEXP and UTEXP performed 4x60 second isometric thumb intervals separated by 60 second rest intervals in a single-blinded placebo-controlled study. Participants were assessed over a 60 minute post-intervention recovery period for pain, blood lactate and lymphocyte subpopulation counts. Results: WLPLA did not change for any measured variable (p>0.05). The two experimentalgroups increased significantly (p<0.01) in thumb pain post-intervention (WLEXP:4.92/10; UTEXP:2.92/10) however only WLEXP remained elevated across all time-points. Blood lactate increased for both experimental groups post-intervention (p<0.01) whilst peak concentrations (UTEXP: 2.2mmol/L; WLEXP: 2.4mmol/L) and temporal profiles were not different between groups (p>0.05). No differences in cell count were seen for CD56+/CD16+ lymphocytes across time for any group (p>0.05). UTEXP showed an early significant increase (20 min post-intervention) in CD4+CD3+ (20.78%, p<0.01), CD8+CD3+ (15.25%, p<0.01) and CD19+ (18.11%, p=0.013) cell count before returning to levels not different from baseline by the final time-point (p>0.05). Conversely, WLEXP group showed no early increase followed by a delayed increase in cell count evident at the final time-point; CD4+CD3+ (19.06%, p<0.01), CD8+CD3+ (11.46%, p=0.033) and CD19+ (28.87%, p<0.01). Blood lactate was not correlated with lymphocyte counts. Conclusions: Physical aptitude and not cellular energy demand influences the lymphocyte response to resistance-exercise.
Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalInternational Journal of Kinesiology and Sports Science
Volume4
Issue number1
DOIs
Publication statusPublished - 2016

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Lymphocytosis
Aptitude
Thumb
Lactic Acid
B-Lymphocytes
Lymphocyte Subsets
Lymphocyte Count
Lymphocytes
T-Lymphocytes
Cell Count
Placebos
Pain

Cite this

@article{eb6e3bbff2bf4a21b48a7277fd710996,
title = "Isometric thumb exertion induces B cell and T cell lymphocytosis in trained and untrained males: Physical aptitude determines response profiles",
abstract = "Purpose: The present study examined the effect of low-dose thumb exertion on lymphocyte subpopulation trafficking. The potential role of blood lactate in mediating lymphocyte redistribution was also investigated. Methods: 27 male participants (18 weightlifting-trained; 9 untrained) were separated into 3 groups of 9 (Weightlifting and Untrained Experimental: WLEXP, UTEXP; Weightlifting Placebo: WLPLA). WLEXP and UTEXP performed 4x60 second isometric thumb intervals separated by 60 second rest intervals in a single-blinded placebo-controlled study. Participants were assessed over a 60 minute post-intervention recovery period for pain, blood lactate and lymphocyte subpopulation counts. Results: WLPLA did not change for any measured variable (p>0.05). The two experimentalgroups increased significantly (p<0.01) in thumb pain post-intervention (WLEXP:4.92/10; UTEXP:2.92/10) however only WLEXP remained elevated across all time-points. Blood lactate increased for both experimental groups post-intervention (p<0.01) whilst peak concentrations (UTEXP: 2.2mmol/L; WLEXP: 2.4mmol/L) and temporal profiles were not different between groups (p>0.05). No differences in cell count were seen for CD56+/CD16+ lymphocytes across time for any group (p>0.05). UTEXP showed an early significant increase (20 min post-intervention) in CD4+CD3+ (20.78{\%}, p<0.01), CD8+CD3+ (15.25{\%}, p<0.01) and CD19+ (18.11{\%}, p=0.013) cell count before returning to levels not different from baseline by the final time-point (p>0.05). Conversely, WLEXP group showed no early increase followed by a delayed increase in cell count evident at the final time-point; CD4+CD3+ (19.06{\%}, p<0.01), CD8+CD3+ (11.46{\%}, p=0.033) and CD19+ (28.87{\%}, p<0.01). Blood lactate was not correlated with lymphocyte counts. Conclusions: Physical aptitude and not cellular energy demand influences the lymphocyte response to resistance-exercise.",
author = "Szlezak, {Adam Michael} and Lotti Tajouri and James Keane and Lauluten, {Siri Lauleten} and Clare Minahan",
year = "2016",
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language = "English",
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pages = "55--66",
journal = "International Journal of Kinesiology and Sports Science",
issn = "2202-946X",
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Isometric thumb exertion induces B cell and T cell lymphocytosis in trained and untrained males : Physical aptitude determines response profiles. / Szlezak, Adam Michael; Tajouri, Lotti; Keane, James; Lauluten, Siri Lauleten; Minahan, Clare.

In: International Journal of Kinesiology and Sports Science, Vol. 4, No. 1, 2016, p. 55-66.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Isometric thumb exertion induces B cell and T cell lymphocytosis in trained and untrained males

T2 - Physical aptitude determines response profiles

AU - Szlezak, Adam Michael

AU - Tajouri, Lotti

AU - Keane, James

AU - Lauluten, Siri Lauleten

AU - Minahan, Clare

PY - 2016

Y1 - 2016

N2 - Purpose: The present study examined the effect of low-dose thumb exertion on lymphocyte subpopulation trafficking. The potential role of blood lactate in mediating lymphocyte redistribution was also investigated. Methods: 27 male participants (18 weightlifting-trained; 9 untrained) were separated into 3 groups of 9 (Weightlifting and Untrained Experimental: WLEXP, UTEXP; Weightlifting Placebo: WLPLA). WLEXP and UTEXP performed 4x60 second isometric thumb intervals separated by 60 second rest intervals in a single-blinded placebo-controlled study. Participants were assessed over a 60 minute post-intervention recovery period for pain, blood lactate and lymphocyte subpopulation counts. Results: WLPLA did not change for any measured variable (p>0.05). The two experimentalgroups increased significantly (p<0.01) in thumb pain post-intervention (WLEXP:4.92/10; UTEXP:2.92/10) however only WLEXP remained elevated across all time-points. Blood lactate increased for both experimental groups post-intervention (p<0.01) whilst peak concentrations (UTEXP: 2.2mmol/L; WLEXP: 2.4mmol/L) and temporal profiles were not different between groups (p>0.05). No differences in cell count were seen for CD56+/CD16+ lymphocytes across time for any group (p>0.05). UTEXP showed an early significant increase (20 min post-intervention) in CD4+CD3+ (20.78%, p<0.01), CD8+CD3+ (15.25%, p<0.01) and CD19+ (18.11%, p=0.013) cell count before returning to levels not different from baseline by the final time-point (p>0.05). Conversely, WLEXP group showed no early increase followed by a delayed increase in cell count evident at the final time-point; CD4+CD3+ (19.06%, p<0.01), CD8+CD3+ (11.46%, p=0.033) and CD19+ (28.87%, p<0.01). Blood lactate was not correlated with lymphocyte counts. Conclusions: Physical aptitude and not cellular energy demand influences the lymphocyte response to resistance-exercise.

AB - Purpose: The present study examined the effect of low-dose thumb exertion on lymphocyte subpopulation trafficking. The potential role of blood lactate in mediating lymphocyte redistribution was also investigated. Methods: 27 male participants (18 weightlifting-trained; 9 untrained) were separated into 3 groups of 9 (Weightlifting and Untrained Experimental: WLEXP, UTEXP; Weightlifting Placebo: WLPLA). WLEXP and UTEXP performed 4x60 second isometric thumb intervals separated by 60 second rest intervals in a single-blinded placebo-controlled study. Participants were assessed over a 60 minute post-intervention recovery period for pain, blood lactate and lymphocyte subpopulation counts. Results: WLPLA did not change for any measured variable (p>0.05). The two experimentalgroups increased significantly (p<0.01) in thumb pain post-intervention (WLEXP:4.92/10; UTEXP:2.92/10) however only WLEXP remained elevated across all time-points. Blood lactate increased for both experimental groups post-intervention (p<0.01) whilst peak concentrations (UTEXP: 2.2mmol/L; WLEXP: 2.4mmol/L) and temporal profiles were not different between groups (p>0.05). No differences in cell count were seen for CD56+/CD16+ lymphocytes across time for any group (p>0.05). UTEXP showed an early significant increase (20 min post-intervention) in CD4+CD3+ (20.78%, p<0.01), CD8+CD3+ (15.25%, p<0.01) and CD19+ (18.11%, p=0.013) cell count before returning to levels not different from baseline by the final time-point (p>0.05). Conversely, WLEXP group showed no early increase followed by a delayed increase in cell count evident at the final time-point; CD4+CD3+ (19.06%, p<0.01), CD8+CD3+ (11.46%, p=0.033) and CD19+ (28.87%, p<0.01). Blood lactate was not correlated with lymphocyte counts. Conclusions: Physical aptitude and not cellular energy demand influences the lymphocyte response to resistance-exercise.

U2 - 10.7575/aiac.ijkss.v.4n.1p.55

DO - 10.7575/aiac.ijkss.v.4n.1p.55

M3 - Article

VL - 4

SP - 55

EP - 66

JO - International Journal of Kinesiology and Sports Science

JF - International Journal of Kinesiology and Sports Science

SN - 2202-946X

IS - 1

ER -