Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

I Pieper, Rachel Smith, Joanna Bishop, O Aldalati, A Chase, Gareth Morgan, Catherine A. Thornton

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood. MSCs were analyzed by trilineage differentiation and flow cytometry. PB MSCs were isolated from 83% of patients (n = 6) but not from controls. The use of PL was feasible in the first passage but not in subsequent ones due to volume. To conclude, PB is a promising alternative to bone marrow. It negates the need for invasive harvesting techniques, and reduces hemorrhagic risk in this patient population routinely managed with anticoagulant and antiplatelet agents.

Original languageEnglish
Article number41
Pages (from-to)654-666
Number of pages13
JournalArtificial Organs
Volume41
Issue number7
Early online date28 Feb 2017
DOIs
Publication statusPublished - 1 Jul 2017

Fingerprint

Mesenchymal Stromal Cells
Blood
Platelets
Bone
Blood Platelets
Flow cytometry
Platelet Aggregation Inhibitors
Bone Marrow
Anticoagulants
Feasibility Studies
ST Elevation Myocardial Infarction
Flow Cytometry
Therapeutics
Myocardial Infarction
Population

Cite this

Pieper, I ; Smith, Rachel ; Bishop, Joanna ; Aldalati, O ; Chase, A ; Morgan, Gareth ; Thornton, Catherine A. / Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients. In: Artificial Organs. 2017 ; Vol. 41, No. 7. pp. 654-666.
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abstract = "Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood. MSCs were analyzed by trilineage differentiation and flow cytometry. PB MSCs were isolated from 83{\%} of patients (n = 6) but not from controls. The use of PL was feasible in the first passage but not in subsequent ones due to volume. To conclude, PB is a promising alternative to bone marrow. It negates the need for invasive harvesting techniques, and reduces hemorrhagic risk in this patient population routinely managed with anticoagulant and antiplatelet agents.",
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Pieper, I, Smith, R, Bishop, J, Aldalati, O, Chase, A, Morgan, G & Thornton, CA 2017, 'Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients' Artificial Organs, vol. 41, no. 7, 41, pp. 654-666. https://doi.org/10.1111/aor.12829

Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients. / Pieper, I; Smith, Rachel; Bishop, Joanna ; Aldalati, O; Chase, A; Morgan, Gareth; Thornton, Catherine A.

In: Artificial Organs, Vol. 41, No. 7, 41, 01.07.2017, p. 654-666.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Smith, Rachel

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AU - Morgan, Gareth

AU - Thornton, Catherine A.

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