Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

I Pieper; Rachel Smith; Joanna  Bishop; O Aldalati; A Chase; Gareth Morgan; Catherine A. Thornton

doi:10.1111/aor.12829

Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

I Pieper, Rachel Smith, Joanna Bishop, O Aldalati, A Chase, Gareth Morgan, Catherine A. Thornton

Faculty of Health Sciences & Medicine

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood. MSCs were analyzed by trilineage differentiation and flow cytometry. PB MSCs were isolated from 83% of patients (n = 6) but not from controls. The use of PL was feasible in the first passage but not in subsequent ones due to volume. To conclude, PB is a promising alternative to bone marrow. It negates the need for invasive harvesting techniques, and reduces hemorrhagic risk in this patient population routinely managed with anticoagulant and antiplatelet agents.

Original language	English
Article number	41
Pages (from-to)	654-666
Number of pages	13
Journal	Artificial Organs
Volume	41
Issue number	7
Early online date	28 Feb 2017
DOIs	https://doi.org/10.1111/aor.12829
Publication status	Published - 1 Jul 2017

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abstract = "Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood. MSCs were analyzed by trilineage differentiation and flow cytometry. PB MSCs were isolated from 83% of patients (n = 6) but not from controls. The use of PL was feasible in the first passage but not in subsequent ones due to volume. To conclude, PB is a promising alternative to bone marrow. It negates the need for invasive harvesting techniques, and reduces hemorrhagic risk in this patient population routinely managed with anticoagulant and antiplatelet agents.",

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AU - Pieper, I

AU - Smith, Rachel

AU - Bishop, Joanna

AU - Aldalati, O

AU - Chase, A

AU - Morgan, Gareth

AU - Thornton, Catherine A.

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Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

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