Investigation of salivary sialic acid as a biomarker in obesity: determination of both free and protein-bound sialic acids 5-N-acetylneuraminic (Neu5Ac) and 5-N-glycolylneuraminic (Neu5Gc) acids in human saliva

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Abstract

Sialic acids are a group of monosaccharides that are abundantly found in the mammalian cell surface. There is a strong correlation between serum sialic acids and various obesity-induced complications, however salivary sialic acids as a biomarker for obesity is a largely unexplored area.
A cost-effective method was developed using reverse-phase ion-pairing HPLC-UV using triisopropylamine as the ionpairing agent with C18 column. Neu5Gc and Neu5Ac were eluted with the retention time of 5.33 min and 5.94 min
respectively with a flow rate of 0.4 mL/min. For Neu5Ac, the recovery from spiking ranged from 79.9% to 109.9% with the limit of detection and limit of quantification calculated for Neu5Ac were 0.0353 mM and 0.1176 mM
respectively.
The column efficiency is 6503 ± 95.22. With optimised method, a possible relationship between total salivary sialic acids level and obesity features such as BMI and fat mass vefore and after the weight loss was explored. Salivary
samples were collected prior to and after the 30 weeks weight loss by calorie restriction from 15 obese women (preBMI: 94.2 kg/m2 , 79.9 – 127.7 kg/m2
, postBMI: 86.4 kg/m2 , 75.2 – 120.2 kg/m2 ). There was no statistically
significant difference in salivary sialic acids level between pre- and post-weight loss (Z = -0.785, p = 0.433) despite significant difference in BMI (Z = -3.408, p = 0.001). There was no statistically significant association between salivary
sialic acids level and obesity features such as BMI (rpre = -0.108, ppre = 0.713; rpost = -0.037, ppost = 0.899) and fat masses as well (rpre = -0.051, ppre = 0.863; rpost = 0.451, ppost = 0.106). The findings from this project mmay add
another viewpoint to previous studies where it was concluded that serum sialic acid level is associated with individual features of metabolic syndrome from obese individuals (Browning et al. 2004).
The work conducted successfully quantified the Neu5Ac levels in human saliva. The exploration of the relationship between sialic acid and obesity was attempted.
Original languageEnglish
Pages17
Number of pages1
Publication statusPublished - 23 Nov 2018
Event3rd Queensland Annual Chemistry Symposium QACS 2018 - Griffith University Nathan Campus, Brisbane, Australia
Duration: 23 Nov 201823 Nov 2018
Conference number: 3rd

Conference

Conference3rd Queensland Annual Chemistry Symposium QACS 2018
Abbreviated titleQACS
CountryAustralia
CityBrisbane
Period23/11/1823/11/18

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