Investigation of murine spleen as a niche for hematopoiesis

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Abstract

Background. Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been hypothesized not to share a bone marrow origin.

Methods. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues.

Results. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed.

Conclusion. Evidence for the maintenance of do-nor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were one of the several DC and myeloid subsets, including activated DC, resembling L-DC. Overall, this study adds further evidence that spleen can support endogenous myelopoiesis.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalTransplantation
Volume89
Issue number2
DOIs
Publication statusPublished - 27 Jan 2010
Externally publishedYes

Cite this

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title = "Investigation of murine spleen as a niche for hematopoiesis",
abstract = "Background. Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely {"}dendritic-like cells{"} (L-DC), has been hypothesized not to share a bone marrow origin.Methods. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues.Results. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed.Conclusion. Evidence for the maintenance of do-nor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were one of the several DC and myeloid subsets, including activated DC, resembling L-DC. Overall, this study adds further evidence that spleen can support endogenous myelopoiesis.",
author = "Tan, {Jonathan K. H.} and O'Neill, {Helen C.}",
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Investigation of murine spleen as a niche for hematopoiesis. / Tan, Jonathan K. H.; O'Neill, Helen C.

In: Transplantation, Vol. 89, No. 2, 27.01.2010, p. 140-145.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Investigation of murine spleen as a niche for hematopoiesis

AU - Tan, Jonathan K. H.

AU - O'Neill, Helen C.

PY - 2010/1/27

Y1 - 2010/1/27

N2 - Background. Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been hypothesized not to share a bone marrow origin.Methods. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues.Results. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed.Conclusion. Evidence for the maintenance of do-nor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were one of the several DC and myeloid subsets, including activated DC, resembling L-DC. Overall, this study adds further evidence that spleen can support endogenous myelopoiesis.

AB - Background. Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been hypothesized not to share a bone marrow origin.Methods. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues.Results. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed.Conclusion. Evidence for the maintenance of do-nor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were one of the several DC and myeloid subsets, including activated DC, resembling L-DC. Overall, this study adds further evidence that spleen can support endogenous myelopoiesis.

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JO - Transplantation bulletin

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SN - 0041-1337

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