Investigation of a neuronal nitric oxide synthase gene (NOS1) polymorphism in a multiple sclerosis population

Lotti Tajouri, Linda Ferreira, Micky Ovcaric, Rob Curtain, Rod A. Lea, Peter A Csurhes, Michael P. Pender, Lyn R. Griffiths

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Abstract

Multiple Sclerosis (MS) is a chronic neurological disease characterized by demyelination associated with infiltrating white blood cells in the central nervous system (CNS). Nitric oxide synthases (NOS) are a family of enzymes that control the production of nitric oxide. It is possible that neuronal NOS could be involved in MS pathophysiology and hence the nNOS gene is a potential candidate for involvement in disease susceptibility. The aim of this study was to determine whether allelic variation at the nNOS gene locus is associated with MS in an Australian cohort. DNA samples obtained from a Caucasian Australian population affected with MS and an unaffected control population, matched for gender, age and ethnicity, were genotyped for a microsatellite polymorphism in the promoter region of the nNOS gene. Allele frequencies were compared using chi-squared based statistical analyses with significance tested by Monte Carlo simulation. Allelic analysis of MS cases and controls produced a chi-squared value of 5.63 with simulated P=0.96 (OR(max)=1.41, 95% CI: 0.926-2.15). Similarly, a Mann-Whitney U analysis gave a non-significant P-value of 0.377 for allele distribution. No differences in allele frequencies were observed for gender or clinical course subtype (P>0.05). Statistical analysis indicated that there is no association of this nNOS variant and MS and hence the gene does not appear to play a genetically significant role in disease susceptibility.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalJournal of the Neurological Sciences
Volume218
Issue number1-2
DOIs
Publication statusPublished - 15 Mar 2004
Externally publishedYes

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Nitric Oxide Synthase Type I
Multiple Sclerosis
Population
Genes
Disease Susceptibility
Gene Frequency
Demyelinating Diseases
Genetic Promoter Regions
Nitric Oxide Synthase
Microsatellite Repeats
Nitric Oxide
Leukocytes
Chronic Disease
Central Nervous System
Alleles
DNA
Enzymes

Cite this

Tajouri, Lotti ; Ferreira, Linda ; Ovcaric, Micky ; Curtain, Rob ; Lea, Rod A. ; Csurhes, Peter A ; Pender, Michael P. ; Griffiths, Lyn R. / Investigation of a neuronal nitric oxide synthase gene (NOS1) polymorphism in a multiple sclerosis population. In: Journal of the Neurological Sciences. 2004 ; Vol. 218, No. 1-2. pp. 25-28.
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Investigation of a neuronal nitric oxide synthase gene (NOS1) polymorphism in a multiple sclerosis population. / Tajouri, Lotti; Ferreira, Linda; Ovcaric, Micky; Curtain, Rob; Lea, Rod A.; Csurhes, Peter A; Pender, Michael P.; Griffiths, Lyn R.

In: Journal of the Neurological Sciences, Vol. 218, No. 1-2, 15.03.2004, p. 25-28.

Research output: Contribution to journalArticleResearchpeer-review

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