Investigation into the prevalence of a novel dendritic-like cell subset in vivo

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Abstract

A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8(+) T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the spleen and ~5% in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T-cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L-DC or an altered phenotype reflective of the phenotype of the mouse strain.

Original languageEnglish
Pages (from-to)1608-18
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Volume17
Issue number12
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

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Dendritic Cells
Spleen
Myeloid Cells
Mutant Strains Mice
Myelopoiesis
CD8 Antigens
T-Lymphocytes
Phenotype
Population
Bone Marrow

Cite this

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title = "Investigation into the prevalence of a novel dendritic-like cell subset in vivo",
abstract = "A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8(+) T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6{\%} of all dendritic and myeloid cells in the spleen and ~5{\%} in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T-cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L-DC or an altered phenotype reflective of the phenotype of the mouse strain.",
author = "Griffiths, {Kristin Lisa} and Tan, {Jonathan Kah Huat} and O'Neill, {Helen Christine}",
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Investigation into the prevalence of a novel dendritic-like cell subset in vivo. / Griffiths, Kristin Lisa; Tan, Jonathan Kah Huat; O'Neill, Helen Christine.

In: Journal of Cellular and Molecular Medicine, Vol. 17, No. 12, 12.2013, p. 1608-18.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Griffiths, Kristin Lisa

AU - Tan, Jonathan Kah Huat

AU - O'Neill, Helen Christine

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AB - A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8(+) T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the spleen and ~5% in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T-cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L-DC or an altered phenotype reflective of the phenotype of the mouse strain.

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