Investigating myeloid development in the context of a spleen microenvironment

Research output: Contribution to journalMeeting AbstractResearchpeer-review

34 Citations (Scopus)

Abstract

The spleen contains multiple myeloid cell populations with varying functions in immunity and wound
healing. The differentiation of hematopoietic stem cells (HSC) and progenitors into myeloid
CD11b+
CD11clo antigen presenting cells (APC) has been previously demonstrated in vitro in the
context of a spleen stromal microenvironment. This same subset is also produced indefinitely in
spleen long-term cultures (LTC), suggesting they represent a spleen endogenous immune cell
population derived from tissue-resident HSC. However, the precise correlation between culturederived myeloid APC and naturally-occurring APC in spleen has been difficult to establish, although
they are clearly distinct from conventional dendritic cells. This study now addresses whether common
monocyte progenitors (CMoP) differentiate to give this distinct subset of myeloid CD11b+
CD11clo APC
in the context of a spleen stromal microenvironment, and whether these represent monocytes or a
unique cell lineage. Findings will be presented demonstrating in vitro spleen stromal co-cultures of
CMoP from sources including adult bone marrow, and adult and neonatal spleen. Co-cultures of HSC
over spleen stroma, and cytokine-induced (SCF, LIF, IL-3 and IL-6) cultures of CMoP, will serve as
controls which produce myeloid CD11b+
CD11clo APC and CD11b+
Ly6Chi monocytes, respectively.
Results from this study specifically allow the establishment of a relationship between co-culture and
LTC-derived myeloid-APC, and cells arising from the monocyte lineage defined by the CMoP
precursor.
Original languageEnglish
Article number984
Pages (from-to)954-954
Number of pages1
JournalEuropean Journal of Immunology
Volume46
Issue numberS1
DOIs
Publication statusPublished - Aug 2016
EventInternational Congress of Immunology (ICI) - Melbourne, Australia
Duration: 21 Aug 201626 Aug 2016

Cite this

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title = "Investigating myeloid development in the context of a spleen microenvironment",
abstract = "The spleen contains multiple myeloid cell populations with varying functions in immunity and woundhealing. The differentiation of hematopoietic stem cells (HSC) and progenitors into myeloidCD11b+CD11clo antigen presenting cells (APC) has been previously demonstrated in vitro in thecontext of a spleen stromal microenvironment. This same subset is also produced indefinitely inspleen long-term cultures (LTC), suggesting they represent a spleen endogenous immune cellpopulation derived from tissue-resident HSC. However, the precise correlation between culturederived myeloid APC and naturally-occurring APC in spleen has been difficult to establish, althoughthey are clearly distinct from conventional dendritic cells. This study now addresses whether commonmonocyte progenitors (CMoP) differentiate to give this distinct subset of myeloid CD11b+CD11clo APCin the context of a spleen stromal microenvironment, and whether these represent monocytes or aunique cell lineage. Findings will be presented demonstrating in vitro spleen stromal co-cultures ofCMoP from sources including adult bone marrow, and adult and neonatal spleen. Co-cultures of HSCover spleen stroma, and cytokine-induced (SCF, LIF, IL-3 and IL-6) cultures of CMoP, will serve ascontrols which produce myeloid CD11b+CD11clo APC and CD11b+Ly6Chi monocytes, respectively.Results from this study specifically allow the establishment of a relationship between co-culture andLTC-derived myeloid-APC, and cells arising from the monocyte lineage defined by the CMoPprecursor.",
author = "C. Short and O'Neill, {Helen C} and J. Tan",
year = "2016",
month = "8",
doi = "10.1002/eji.201670200",
language = "English",
volume = "46",
pages = "954--954",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-Blackwell",
number = "S1",

}

Investigating myeloid development in the context of a spleen microenvironment. / Short, C.; O'Neill, Helen C; Tan, J.

In: European Journal of Immunology, Vol. 46, No. S1, 984, 08.2016, p. 954-954.

Research output: Contribution to journalMeeting AbstractResearchpeer-review

TY - JOUR

T1 - Investigating myeloid development in the context of a spleen microenvironment

AU - Short, C.

AU - O'Neill, Helen C

AU - Tan, J.

PY - 2016/8

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N2 - The spleen contains multiple myeloid cell populations with varying functions in immunity and woundhealing. The differentiation of hematopoietic stem cells (HSC) and progenitors into myeloidCD11b+CD11clo antigen presenting cells (APC) has been previously demonstrated in vitro in thecontext of a spleen stromal microenvironment. This same subset is also produced indefinitely inspleen long-term cultures (LTC), suggesting they represent a spleen endogenous immune cellpopulation derived from tissue-resident HSC. However, the precise correlation between culturederived myeloid APC and naturally-occurring APC in spleen has been difficult to establish, althoughthey are clearly distinct from conventional dendritic cells. This study now addresses whether commonmonocyte progenitors (CMoP) differentiate to give this distinct subset of myeloid CD11b+CD11clo APCin the context of a spleen stromal microenvironment, and whether these represent monocytes or aunique cell lineage. Findings will be presented demonstrating in vitro spleen stromal co-cultures ofCMoP from sources including adult bone marrow, and adult and neonatal spleen. Co-cultures of HSCover spleen stroma, and cytokine-induced (SCF, LIF, IL-3 and IL-6) cultures of CMoP, will serve ascontrols which produce myeloid CD11b+CD11clo APC and CD11b+Ly6Chi monocytes, respectively.Results from this study specifically allow the establishment of a relationship between co-culture andLTC-derived myeloid-APC, and cells arising from the monocyte lineage defined by the CMoPprecursor.

AB - The spleen contains multiple myeloid cell populations with varying functions in immunity and woundhealing. The differentiation of hematopoietic stem cells (HSC) and progenitors into myeloidCD11b+CD11clo antigen presenting cells (APC) has been previously demonstrated in vitro in thecontext of a spleen stromal microenvironment. This same subset is also produced indefinitely inspleen long-term cultures (LTC), suggesting they represent a spleen endogenous immune cellpopulation derived from tissue-resident HSC. However, the precise correlation between culturederived myeloid APC and naturally-occurring APC in spleen has been difficult to establish, althoughthey are clearly distinct from conventional dendritic cells. This study now addresses whether commonmonocyte progenitors (CMoP) differentiate to give this distinct subset of myeloid CD11b+CD11clo APCin the context of a spleen stromal microenvironment, and whether these represent monocytes or aunique cell lineage. Findings will be presented demonstrating in vitro spleen stromal co-cultures ofCMoP from sources including adult bone marrow, and adult and neonatal spleen. Co-cultures of HSCover spleen stroma, and cytokine-induced (SCF, LIF, IL-3 and IL-6) cultures of CMoP, will serve ascontrols which produce myeloid CD11b+CD11clo APC and CD11b+Ly6Chi monocytes, respectively.Results from this study specifically allow the establishment of a relationship between co-culture andLTC-derived myeloid-APC, and cells arising from the monocyte lineage defined by the CMoPprecursor.

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DO - 10.1002/eji.201670200

M3 - Meeting Abstract

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JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

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