Interaction of contractile activity and training history on mRNA abundance in skeletal muscle from trained athletes

Vernon G. Coffey, Anthony J. Shield, Benedict J. Canny, Kate A. Carey, David Cameron-Smith, John A. Hawley

Research output: Contribution to journalArticleResearchpeer-review

97 Citations (Scopus)

Abstract

Skeletal muscle displays enormous plasticity to respond to contractile activity with muscle from strength- (ST) and endurance-trained (ET) athletes representing diverse states of the adaptation continuum. Training adaptation can be viewed as the accumulation of specific proteins. Hence, the altered gene expression that allows for changes in protein concentration is of major importance for any training adaptation. Accordingly, the aim of the present study was to quantify acute subcellular responses in muscle to habitual and unfamiliar exercise. After 24-h diet/exercise control, 13 male subjects (7 ST and 6 ET) performed a random order of either resistance (8 × 5 maximal leg extensions) or endurance exercise (1 h of cycling at 70% peak O 2 uptake). Muscle biopsies were taken from vastus lateralis at rest and 3 h after exercise. Gene expression was analyzed using real-time PCR with changes normalized relative to preexercise values. After cycling exercise, peroxisome proliferator-activated receptor-γ coactivator-1α (ET ∼8.5-fold, ST ∼10-fold, P < 0.001), pyruvate dehydrogenase kinase-4 (PDK-4; ET ∼26-fold, ST ∼39-fold), vascular endothelial growth factor (VEGF; ET ∼4.5-fold, ST ∼4-fold), and muscle atrophy F-box protein (MAFbx) (ET ∼2-fold, ST ∼0.4-fold) mRNA increased in both groups, whereas MyoD (∼3-fold), myogenin (∼0.9-fold), and myostatin (∼2-fold) mRNA increased in ET but not in ST (P < 0.05). After resistance exercise PDK-4 (∼7-fold, P < 0.01) and MyoD (∼0.7-fold) increased, whereas MAFbx (∼0.7-fold) and myostatin (∼0.6-fold) decreased in ET but not in ST. We conclude that prior training history can modify the acute gene responses in skeletal muscle to subsequent exercise.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume290
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Fingerprint

Athletes
Muscle
Teaching
Skeletal Muscle
Durability
History
Exercise
Messenger RNA
F-Box Proteins
Myostatin
Muscular Atrophy
Vascular Endothelial Growth Factor A
Myogenin
Gene Expression
Gene expression
Muscles
Peroxisome Proliferator-Activated Receptors
Quadriceps Muscle
Muscle Strength
Real-Time Polymerase Chain Reaction

Cite this

Coffey, Vernon G. ; Shield, Anthony J. ; Canny, Benedict J. ; Carey, Kate A. ; Cameron-Smith, David ; Hawley, John A. / Interaction of contractile activity and training history on mRNA abundance in skeletal muscle from trained athletes. In: American Journal of Physiology - Endocrinology and Metabolism. 2006 ; Vol. 290, No. 5.
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abstract = "Skeletal muscle displays enormous plasticity to respond to contractile activity with muscle from strength- (ST) and endurance-trained (ET) athletes representing diverse states of the adaptation continuum. Training adaptation can be viewed as the accumulation of specific proteins. Hence, the altered gene expression that allows for changes in protein concentration is of major importance for any training adaptation. Accordingly, the aim of the present study was to quantify acute subcellular responses in muscle to habitual and unfamiliar exercise. After 24-h diet/exercise control, 13 male subjects (7 ST and 6 ET) performed a random order of either resistance (8 × 5 maximal leg extensions) or endurance exercise (1 h of cycling at 70{\%} peak O 2 uptake). Muscle biopsies were taken from vastus lateralis at rest and 3 h after exercise. Gene expression was analyzed using real-time PCR with changes normalized relative to preexercise values. After cycling exercise, peroxisome proliferator-activated receptor-γ coactivator-1α (ET ∼8.5-fold, ST ∼10-fold, P < 0.001), pyruvate dehydrogenase kinase-4 (PDK-4; ET ∼26-fold, ST ∼39-fold), vascular endothelial growth factor (VEGF; ET ∼4.5-fold, ST ∼4-fold), and muscle atrophy F-box protein (MAFbx) (ET ∼2-fold, ST ∼0.4-fold) mRNA increased in both groups, whereas MyoD (∼3-fold), myogenin (∼0.9-fold), and myostatin (∼2-fold) mRNA increased in ET but not in ST (P < 0.05). After resistance exercise PDK-4 (∼7-fold, P < 0.01) and MyoD (∼0.7-fold) increased, whereas MAFbx (∼0.7-fold) and myostatin (∼0.6-fold) decreased in ET but not in ST. We conclude that prior training history can modify the acute gene responses in skeletal muscle to subsequent exercise.",
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Interaction of contractile activity and training history on mRNA abundance in skeletal muscle from trained athletes. / Coffey, Vernon G.; Shield, Anthony J.; Canny, Benedict J.; Carey, Kate A.; Cameron-Smith, David; Hawley, John A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 290, No. 5, 05.2006.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Shield, Anthony J.

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AU - Hawley, John A.

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