Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo

E. Corpeleijn, M. M.A.L. Pelsers, S. Soenen, M. Mensink, F. G. Bouwman, M. E. Kooi, W. H.M. Saris, J. F.C. Glatz, E. E. Blaak

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Abstract

Enhanced fatty acid uptake may lead to the accumulation of lipid intermediates. This is related to insulin resistance and type 2 diabetes mellitus. Rodent studies suggest that fatty acid transporters are acutely regulated by insulin. We investigated differences in fatty acid transporter content before and at the end of a hyperinsulinemic euglycemic clamp in skeletal muscle (m. vastus lateralis) of obese, glucose-intolerant men (IGT) and obese normal glucose tolerant controls (NGT). The fatty acid transporter FAT/CD36 protein content increased 1.5-fold (P < 0.05) after 3-hrs of insulin stimulation with no difference between IGT and control subjects. No change was seen in cytosolic fatty acid binding protein (FABPc) protein content. The increase in FAT/CD36 protein content was positively related to insulin resistance as measured during the clamp (r = 0.56, P < 0.05). An increase in FAT/CD36 protein content in skeletal muscle may result in a higher fractional extraction of fatty acids (larger relative uptake) after a meal, enhancing triglyceride accumulation in the muscle. We conclude that also in obese humans the FAT/CD36 protein content in skeletal muscle is dynamically regulated by insulin in vivo on the short term.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalJournal of Physiology and Pharmacology
Volume59
Issue number1
Publication statusPublished - 1 Mar 2008
Externally publishedYes

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CD36 Antigens
Skeletal Muscle
Up-Regulation
Fatty Acids
Insulin
Proteins
Insulin Resistance
Glucose
Fatty Acid-Binding Proteins
Glucose Clamp Technique
Quadriceps Muscle
Type 2 Diabetes Mellitus
Meals
Rodentia
Triglycerides
Lipids
Muscles

Cite this

Corpeleijn, E., Pelsers, M. M. A. L., Soenen, S., Mensink, M., Bouwman, F. G., Kooi, M. E., ... Blaak, E. E. (2008). Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo. Journal of Physiology and Pharmacology, 59(1), 77-83.
Corpeleijn, E. ; Pelsers, M. M.A.L. ; Soenen, S. ; Mensink, M. ; Bouwman, F. G. ; Kooi, M. E. ; Saris, W. H.M. ; Glatz, J. F.C. ; Blaak, E. E. / Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo. In: Journal of Physiology and Pharmacology. 2008 ; Vol. 59, No. 1. pp. 77-83.
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Corpeleijn, E, Pelsers, MMAL, Soenen, S, Mensink, M, Bouwman, FG, Kooi, ME, Saris, WHM, Glatz, JFC & Blaak, EE 2008, 'Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo' Journal of Physiology and Pharmacology, vol. 59, no. 1, pp. 77-83.

Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo. / Corpeleijn, E.; Pelsers, M. M.A.L.; Soenen, S.; Mensink, M.; Bouwman, F. G.; Kooi, M. E.; Saris, W. H.M.; Glatz, J. F.C.; Blaak, E. E.

In: Journal of Physiology and Pharmacology, Vol. 59, No. 1, 01.03.2008, p. 77-83.

Research output: Contribution to journalArticleResearchpeer-review

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