Induction of leukemia in mice using a radiation leukemia virus-induced cell line: A model system for studying oncogenic progression

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Leukemogenesis induced by slowly transforming retroviruses is a multistep process which is difficult to dissect because of its long latency and the problem of distinguishing oncogenic from differentiative events. A method for leukemia induction in mice has been developed using a cell line isolated following in vitro infection with the slowly transforming murine radiation leukemia virus (RadLV). The CI-V13D cell line represents a lymphoid precursor cell type at an early stage in cell transformation and can develop subcutaneous tumors in irradiated syngeneic hosts but not in allogeneic mice even after sublethal irradiation. Selective growth in allogeneic (CBA/H) mouse thymus has been demonstrated, but this requires pre-irradiation of the recipient. Upon reisolation from CBA/H thymus, C1-V13D progeny clones displayed increased tumorigenic potential in comparison to the 'parental' CI-V13D cell line. Tumorigenicity was shown to increase with serial passage through thymus and electron micrographs of clones also revealed increased production of C-type retroviruses. This new model for oncogenic progression should be more amenable to analysis of early genetic changes occurring during replication of leukemia in the thymus.

Original languageEnglish
Pages (from-to)83-93
Number of pages11
JournalLeukemia Research
Volume19
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Radiation Leukemia Virus
Thymus Gland
Leukemia
Cell Line
Retroviridae
Clone Cells
Serial Passage
Murine Leukemia Viruses
Inbred CBA Mouse
Lymphocytes
Electrons
Growth
Infection
Neoplasms

Cite this

@article{3463da488be844a5bd7f30dc35b92186,
title = "Induction of leukemia in mice using a radiation leukemia virus-induced cell line: A model system for studying oncogenic progression",
abstract = "Leukemogenesis induced by slowly transforming retroviruses is a multistep process which is difficult to dissect because of its long latency and the problem of distinguishing oncogenic from differentiative events. A method for leukemia induction in mice has been developed using a cell line isolated following in vitro infection with the slowly transforming murine radiation leukemia virus (RadLV). The CI-V13D cell line represents a lymphoid precursor cell type at an early stage in cell transformation and can develop subcutaneous tumors in irradiated syngeneic hosts but not in allogeneic mice even after sublethal irradiation. Selective growth in allogeneic (CBA/H) mouse thymus has been demonstrated, but this requires pre-irradiation of the recipient. Upon reisolation from CBA/H thymus, C1-V13D progeny clones displayed increased tumorigenic potential in comparison to the 'parental' CI-V13D cell line. Tumorigenicity was shown to increase with serial passage through thymus and electron micrographs of clones also revealed increased production of C-type retroviruses. This new model for oncogenic progression should be more amenable to analysis of early genetic changes occurring during replication of leukemia in the thymus.",
author = "Ho, {Ellen S P} and O'Neill, {Terence J.} and O'Neill, {Helen C.}",
year = "1995",
doi = "10.1016/0145-2126(94)00120-Y",
language = "English",
volume = "19",
pages = "83--93",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier",
number = "2",

}

Induction of leukemia in mice using a radiation leukemia virus-induced cell line : A model system for studying oncogenic progression. / Ho, Ellen S P; O'Neill, Terence J.; O'Neill, Helen C.

In: Leukemia Research, Vol. 19, No. 2, 1995, p. 83-93.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Induction of leukemia in mice using a radiation leukemia virus-induced cell line

T2 - A model system for studying oncogenic progression

AU - Ho, Ellen S P

AU - O'Neill, Terence J.

AU - O'Neill, Helen C.

PY - 1995

Y1 - 1995

N2 - Leukemogenesis induced by slowly transforming retroviruses is a multistep process which is difficult to dissect because of its long latency and the problem of distinguishing oncogenic from differentiative events. A method for leukemia induction in mice has been developed using a cell line isolated following in vitro infection with the slowly transforming murine radiation leukemia virus (RadLV). The CI-V13D cell line represents a lymphoid precursor cell type at an early stage in cell transformation and can develop subcutaneous tumors in irradiated syngeneic hosts but not in allogeneic mice even after sublethal irradiation. Selective growth in allogeneic (CBA/H) mouse thymus has been demonstrated, but this requires pre-irradiation of the recipient. Upon reisolation from CBA/H thymus, C1-V13D progeny clones displayed increased tumorigenic potential in comparison to the 'parental' CI-V13D cell line. Tumorigenicity was shown to increase with serial passage through thymus and electron micrographs of clones also revealed increased production of C-type retroviruses. This new model for oncogenic progression should be more amenable to analysis of early genetic changes occurring during replication of leukemia in the thymus.

AB - Leukemogenesis induced by slowly transforming retroviruses is a multistep process which is difficult to dissect because of its long latency and the problem of distinguishing oncogenic from differentiative events. A method for leukemia induction in mice has been developed using a cell line isolated following in vitro infection with the slowly transforming murine radiation leukemia virus (RadLV). The CI-V13D cell line represents a lymphoid precursor cell type at an early stage in cell transformation and can develop subcutaneous tumors in irradiated syngeneic hosts but not in allogeneic mice even after sublethal irradiation. Selective growth in allogeneic (CBA/H) mouse thymus has been demonstrated, but this requires pre-irradiation of the recipient. Upon reisolation from CBA/H thymus, C1-V13D progeny clones displayed increased tumorigenic potential in comparison to the 'parental' CI-V13D cell line. Tumorigenicity was shown to increase with serial passage through thymus and electron micrographs of clones also revealed increased production of C-type retroviruses. This new model for oncogenic progression should be more amenable to analysis of early genetic changes occurring during replication of leukemia in the thymus.

UR - http://www.scopus.com/inward/record.url?scp=0028935303&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(94)00120-Y

DO - 10.1016/0145-2126(94)00120-Y

M3 - Article

VL - 19

SP - 83

EP - 93

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 2

ER -