In vivo myometrial activity in the rat during the oestro us cycle: Studies with a novel technique of video laparoscopy

Linda H. Crane, Len Martin

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Previous techniques of recording myométrial activity in vivo gave limited information about the nature of contractions, and disrupted normal reproductive events. To overcome these drawbacks we developed a new in vivo method of video laparoscopy (VL). This involves positioning a laparoscope in the abdomen of anaesthetized rats to view the caudal ends of both uterine horns. Myométrial activity is recorded by video camera onto video tape. Myométrial contractions are classified according to the muscle layers involved, the interaction between layers and the direction of propagation. Experiments with intrauterine balloons and electromyography (EMG) in conscious and ketamine/xylazine anaesthetized rats showed that this anaesthetic does not have major effects on myométrial activity. To validate the VL method, recordings were obtained throughout the oestrous cycle and compared with results obtained with EMG in conscious rats. The frequency and pattern of activity were similar with both techniques although more information was obtained from VL. Frequency of contractions was highest in oestrus and dioestrus and lowest in pro-oestrus, when contractions occurred in groups separated by quiescent intervals. At all stages, longitudinal contractions propagating towards the cervix predominated. Circular muscle activity was only seen at oestrus and dioestrus; that at oestrus consisted of weak peristalses, that at dioestrus was more complex. A major advantage of VL is that is does not interfere with the course of the oestrous cycle, pseudopregnancy or early pregnancy.

Original languageEnglish
Pages (from-to)185-199
Number of pages15
JournalReproduction, Fertility and Development
Volume3
Issue number2
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Fingerprint

laparoscopy
Estrus
Diestrus
Laparoscopy
diestrus
estrus
electromyography
rats
Electromyography
estrous cycle
Pseudopregnancy
Laparoscopes
pseudopregnancy
Xylazine
proestrus
Muscles
muscles
video cameras
cervix
xylazine

Cite this

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abstract = "Previous techniques of recording myom{\'e}trial activity in vivo gave limited information about the nature of contractions, and disrupted normal reproductive events. To overcome these drawbacks we developed a new in vivo method of video laparoscopy (VL). This involves positioning a laparoscope in the abdomen of anaesthetized rats to view the caudal ends of both uterine horns. Myom{\'e}trial activity is recorded by video camera onto video tape. Myom{\'e}trial contractions are classified according to the muscle layers involved, the interaction between layers and the direction of propagation. Experiments with intrauterine balloons and electromyography (EMG) in conscious and ketamine/xylazine anaesthetized rats showed that this anaesthetic does not have major effects on myom{\'e}trial activity. To validate the VL method, recordings were obtained throughout the oestrous cycle and compared with results obtained with EMG in conscious rats. The frequency and pattern of activity were similar with both techniques although more information was obtained from VL. Frequency of contractions was highest in oestrus and dioestrus and lowest in pro-oestrus, when contractions occurred in groups separated by quiescent intervals. At all stages, longitudinal contractions propagating towards the cervix predominated. Circular muscle activity was only seen at oestrus and dioestrus; that at oestrus consisted of weak peristalses, that at dioestrus was more complex. A major advantage of VL is that is does not interfere with the course of the oestrous cycle, pseudopregnancy or early pregnancy.",
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In vivo myometrial activity in the rat during the oestro us cycle : Studies with a novel technique of video laparoscopy. / Crane, Linda H.; Martin, Len.

In: Reproduction, Fertility and Development, Vol. 3, No. 2, 01.01.1991, p. 185-199.

Research output: Contribution to journalArticleResearchpeer-review

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