In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species: A study of the production of extracellular metabolites by Trichoderma species

Claudia Calistru, Michelle McLean, Patricia Berjak

Research output: Contribution to journalArticleResearchpeer-review

79 Citations (Scopus)

Abstract

Culture flitrates of Trichoderma viride and Trichoderma harzianum were inhibitory of Fusarium moniliforme and, to a lesser extent, Aspergillus flavus. The degree of inhibition was, however, dependent on the carbon or nitrogen source incorporated into the medium. Scanning electron microscopy revealed the development of abnormal fruiting structures on exposure to some Trichoderma culture filtrate, while macroscopically, growth restriction and, in the case of A. flavus, altered colony colouration were observed. Based on the results of inverted colony culture, it would appear that some isolates of Trichoderma produce inhibitory volatile compounds. The production of possible antibiotics was also demonstrated. The aggressive behaviour (towards A. flavus and F. moniliforme) demonstrated by Trichoderma spp. may be partly explained by the liberation of extracellular enzymes by these fungi. An isolate of T. viride exhibited amylolytic, pectinolytic, proteolytic and cellulolytic activity. Based on the results of the present investigation, Trichoderma spp. are potential candidates for biocontrol of some mycotoxin-producing fungi, but there exists some doubt as to their osmotolerance within the air-dry seed.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalMycopathologia
Volume137
Issue number2
DOIs
Publication statusPublished - 1 Jan 1997
Externally publishedYes

Fingerprint

Aspergillus flavus
Trichoderma
Fusarium
in vitro studies
biological control
metabolites
Trichoderma viride
osmotolerance
fungi
Trichoderma harzianum
abnormal development
culture filtrates
Fungi
mycotoxins
volatile compounds
fruiting
aggression
scanning electron microscopy
Mycotoxins
antibiotics

Cite this

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title = "In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species: A study of the production of extracellular metabolites by Trichoderma species",
abstract = "Culture flitrates of Trichoderma viride and Trichoderma harzianum were inhibitory of Fusarium moniliforme and, to a lesser extent, Aspergillus flavus. The degree of inhibition was, however, dependent on the carbon or nitrogen source incorporated into the medium. Scanning electron microscopy revealed the development of abnormal fruiting structures on exposure to some Trichoderma culture filtrate, while macroscopically, growth restriction and, in the case of A. flavus, altered colony colouration were observed. Based on the results of inverted colony culture, it would appear that some isolates of Trichoderma produce inhibitory volatile compounds. The production of possible antibiotics was also demonstrated. The aggressive behaviour (towards A. flavus and F. moniliforme) demonstrated by Trichoderma spp. may be partly explained by the liberation of extracellular enzymes by these fungi. An isolate of T. viride exhibited amylolytic, pectinolytic, proteolytic and cellulolytic activity. Based on the results of the present investigation, Trichoderma spp. are potential candidates for biocontrol of some mycotoxin-producing fungi, but there exists some doubt as to their osmotolerance within the air-dry seed.",
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In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species : A study of the production of extracellular metabolites by Trichoderma species. / Calistru, Claudia; McLean, Michelle; Berjak, Patricia.

In: Mycopathologia, Vol. 137, No. 2, 01.01.1997, p. 115-124.

Research output: Contribution to journalArticleResearchpeer-review

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