Identifying tissue-resident hematopoietic stem cells in extrameduallary sites

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Endothelial protein C receptor (EPCR) has been established as a marker specifically distinguishing hematopoietic stem cells (HSC) that reside in murine bone marrow. In contrast, HSC released into the peripheral blood circulation do not express EPCR. We questioned whether the use of EPCR could be extended to identifying tissue-resident HSC in extramedullary organs. Murine spleen is known to harbour HSC from neonatal to adult stages. As an organ that functions explicitly in blood filtration, it has been uncertain whether these cells represent transitory or endogenous HSC. Studies using parabiosis have now clarified that a large proportion of spleen HSC are in fact endogenous to the tissue. We now make a direct comparison of adult spleen, peripheral blood, and bone marrow HSC, to determine the percentage of EPCR+, and thus tissue-resident HSC, in spleen. Our results confirm that spleen indeed contains a high percentage of EPCR+ HSC comparable to bone marrow. We also extend our investigation to examine whether several modes of extramedullary hematopoiesis lead to an expansion of spleen-resident EPCR+ HSC, or an influx of HSC mobilised into the circulation from bone marrow.
Original languageEnglish
Publication statusPublished - 3 Jun 2016
EventEuropean Molecular Biology Laboratory Conference Hematopoietic Stem Cells: From the Embryo to the Aging Organism - European Molecular Biology Laboratory, Heidelberg, Germany
Duration: 3 Jun 20165 Jun 2016
https://www.embl.de/training/events/2016/EHT16-01/index.html

Conference

ConferenceEuropean Molecular Biology Laboratory Conference Hematopoietic Stem Cells
Abbreviated titleEMBL
CountryGermany
CityHeidelberg
Period3/06/165/06/16
Internet address

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Hematopoietic Stem Cells
Protein C
Spleen
Bone Marrow
Parabiosis
Extramedullary Hematopoiesis
Blood Circulation

Cite this

Kaden, J., O'Neill, H. C., & Tan, J. K. H. (2016). Identifying tissue-resident hematopoietic stem cells in extrameduallary sites. Abstract from European Molecular Biology Laboratory Conference Hematopoietic Stem Cells, Heidelberg, Germany.
Kaden, Jacqualine ; O'Neill, Helen C ; Tan, Jonathan Kah Huat. / Identifying tissue-resident hematopoietic stem cells in extrameduallary sites. Abstract from European Molecular Biology Laboratory Conference Hematopoietic Stem Cells, Heidelberg, Germany.
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abstract = "Endothelial protein C receptor (EPCR) has been established as a marker specifically distinguishing hematopoietic stem cells (HSC) that reside in murine bone marrow. In contrast, HSC released into the peripheral blood circulation do not express EPCR. We questioned whether the use of EPCR could be extended to identifying tissue-resident HSC in extramedullary organs. Murine spleen is known to harbour HSC from neonatal to adult stages. As an organ that functions explicitly in blood filtration, it has been uncertain whether these cells represent transitory or endogenous HSC. Studies using parabiosis have now clarified that a large proportion of spleen HSC are in fact endogenous to the tissue. We now make a direct comparison of adult spleen, peripheral blood, and bone marrow HSC, to determine the percentage of EPCR+, and thus tissue-resident HSC, in spleen. Our results confirm that spleen indeed contains a high percentage of EPCR+ HSC comparable to bone marrow. We also extend our investigation to examine whether several modes of extramedullary hematopoiesis lead to an expansion of spleen-resident EPCR+ HSC, or an influx of HSC mobilised into the circulation from bone marrow.",
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Kaden, J, O'Neill, HC & Tan, JKH 2016, 'Identifying tissue-resident hematopoietic stem cells in extrameduallary sites' European Molecular Biology Laboratory Conference Hematopoietic Stem Cells, Heidelberg, Germany, 3/06/16 - 5/06/16, .

Identifying tissue-resident hematopoietic stem cells in extrameduallary sites. / Kaden, Jacqualine; O'Neill, Helen C; Tan, Jonathan Kah Huat.

2016. Abstract from European Molecular Biology Laboratory Conference Hematopoietic Stem Cells, Heidelberg, Germany.

Research output: Contribution to conferenceAbstractResearchpeer-review

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T1 - Identifying tissue-resident hematopoietic stem cells in extrameduallary sites

AU - Kaden, Jacqualine

AU - O'Neill, Helen C

AU - Tan, Jonathan Kah Huat

PY - 2016/6/3

Y1 - 2016/6/3

N2 - Endothelial protein C receptor (EPCR) has been established as a marker specifically distinguishing hematopoietic stem cells (HSC) that reside in murine bone marrow. In contrast, HSC released into the peripheral blood circulation do not express EPCR. We questioned whether the use of EPCR could be extended to identifying tissue-resident HSC in extramedullary organs. Murine spleen is known to harbour HSC from neonatal to adult stages. As an organ that functions explicitly in blood filtration, it has been uncertain whether these cells represent transitory or endogenous HSC. Studies using parabiosis have now clarified that a large proportion of spleen HSC are in fact endogenous to the tissue. We now make a direct comparison of adult spleen, peripheral blood, and bone marrow HSC, to determine the percentage of EPCR+, and thus tissue-resident HSC, in spleen. Our results confirm that spleen indeed contains a high percentage of EPCR+ HSC comparable to bone marrow. We also extend our investigation to examine whether several modes of extramedullary hematopoiesis lead to an expansion of spleen-resident EPCR+ HSC, or an influx of HSC mobilised into the circulation from bone marrow.

AB - Endothelial protein C receptor (EPCR) has been established as a marker specifically distinguishing hematopoietic stem cells (HSC) that reside in murine bone marrow. In contrast, HSC released into the peripheral blood circulation do not express EPCR. We questioned whether the use of EPCR could be extended to identifying tissue-resident HSC in extramedullary organs. Murine spleen is known to harbour HSC from neonatal to adult stages. As an organ that functions explicitly in blood filtration, it has been uncertain whether these cells represent transitory or endogenous HSC. Studies using parabiosis have now clarified that a large proportion of spleen HSC are in fact endogenous to the tissue. We now make a direct comparison of adult spleen, peripheral blood, and bone marrow HSC, to determine the percentage of EPCR+, and thus tissue-resident HSC, in spleen. Our results confirm that spleen indeed contains a high percentage of EPCR+ HSC comparable to bone marrow. We also extend our investigation to examine whether several modes of extramedullary hematopoiesis lead to an expansion of spleen-resident EPCR+ HSC, or an influx of HSC mobilised into the circulation from bone marrow.

M3 - Abstract

ER -

Kaden J, O'Neill HC, Tan JKH. Identifying tissue-resident hematopoietic stem cells in extrameduallary sites. 2016. Abstract from European Molecular Biology Laboratory Conference Hematopoietic Stem Cells, Heidelberg, Germany.