Identification of stromal cells in spleen which support myelopoiesis

Hong Lim, Helen C O'Neill

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Stromal cells in spleen organise tissue into red pulp, white pulp and marginal zone and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.
LanguageEnglish
Article number425328
JournalFrontiers in Cell and Developmental Biology
Volume7
Issue number1
DOIs
Publication statusAccepted/In press - 8 Jan 2019

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Myelopoiesis
Stromal Cells
Spleen
Hematopoiesis
Cell Fractionation
Cell Line
Growth

Cite this

Lim, Hong ; O'Neill, Helen C. / Identification of stromal cells in spleen which support myelopoiesis. In: Frontiers in Cell and Developmental Biology. 2019 ; Vol. 7, No. 1.
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Lim, H & O'Neill, HC 2019, 'Identification of stromal cells in spleen which support myelopoiesis' Frontiers in Cell and Developmental Biology, vol. 7, no. 1, 425328. https://doi.org/10.3389/fcell.2019.00001

Identification of stromal cells in spleen which support myelopoiesis. / Lim, Hong; O'Neill, Helen C.

In: Frontiers in Cell and Developmental Biology, Vol. 7, No. 1, 425328, 08.01.2019.

Research output: Contribution to journal › Article › Research › peer-review

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AB - Stromal cells in spleen organise tissue into red pulp, white pulp and marginal zone and also interact with hematopoietic cells to regulate immune responses. This study has used phenotypic information of a previously described spleen stromal cell line called 5G3, which supports restricted hematopoiesis in vitro, to identify an equivalent stromal cell subset in vivo and to test its capacity to support hematopoiesis. Using stromal cell fractionation, phenotypic analysis, as well as cell growth and hematopoietic support assays, the Sca-1+gp38+Thy1.2+CD29+CD51+ fraction of spleen stroma has been identified as an equivalent stromal subset resembling the 5G3 cell counterpart. While heterogeneity may still exist within that subset, it has been shown to have superior hematopoietic support capacity compared with the 5G3 cell line, and all other spleen stromal cell fractions tested.

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Lim H, O'Neill HC. Identification of stromal cells in spleen which support myelopoiesis. Frontiers in Cell and Developmental Biology. 2019 Jan 8;7(1). 425328. https://doi.org/10.3389/fcell.2019.00001

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