Identification of class I H-2Db molecules primarily expressed by B lymphocytes in murine spleen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Evidence is presented which supports the phenomenon of heterogeneity amongst H-2Db-encoded Class I molecules. Two monoclonal antibodies (MoAb) called H141-31 and B22-249 were used in these studies. Both bind to the 'private' H-2.2 site of H-2Db-encoded molecules, but the binding of B22-249 is determined by carbohydrate moieties, whereas H141-31 appears to bind to a protein-defined epitope. Some H-2Db molecules, identified by the H141-31 MoAb, are primarily expressed on B lymphocytes and not T lymphocytes in spleen. The number of H-2Db molecules which bind H141-31 on B cells was also found to be three- to four-fold less than the number which bound the B22-249 MoAb. B cells of two mutant strains of mice, B6-C.H-2bm13 and B6-C.H-2bm14 which harbour very few nucleotide changes in the H-2Db gene, also show marked reduction in the binding of both antibodies. This suggests that a single common gene encodes both target molecules and that post-translational modifications such as differential glycosylation may account for heterogeneity amongst H-2Db molecules. This would explain the presence of the different H-2Db molecules defined here. It follows that differences in glycosylation evidently occur both within the B cell population, since H141-31 binds to only a subset of H-2Db molecules on B cells, and between T and B lymphocytes, since resting T cells do not bind H141-31 MoAb.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalImmunology and Cell Biology
Volume69
Issue number2
DOIs
Publication statusPublished - Apr 1991
Externally publishedYes

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B-Lymphocytes
Spleen
Monoclonal Antibodies
T-Lymphocytes
Glycosylation
Mutant Strains Mice
Post Translational Protein Processing
Genes
Epitopes
Nucleotides
Carbohydrates
Antibodies
Population
Proteins

Cite this

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title = "Identification of class I H-2Db molecules primarily expressed by B lymphocytes in murine spleen",
abstract = "Evidence is presented which supports the phenomenon of heterogeneity amongst H-2Db-encoded Class I molecules. Two monoclonal antibodies (MoAb) called H141-31 and B22-249 were used in these studies. Both bind to the 'private' H-2.2 site of H-2Db-encoded molecules, but the binding of B22-249 is determined by carbohydrate moieties, whereas H141-31 appears to bind to a protein-defined epitope. Some H-2Db molecules, identified by the H141-31 MoAb, are primarily expressed on B lymphocytes and not T lymphocytes in spleen. The number of H-2Db molecules which bind H141-31 on B cells was also found to be three- to four-fold less than the number which bound the B22-249 MoAb. B cells of two mutant strains of mice, B6-C.H-2bm13 and B6-C.H-2bm14 which harbour very few nucleotide changes in the H-2Db gene, also show marked reduction in the binding of both antibodies. This suggests that a single common gene encodes both target molecules and that post-translational modifications such as differential glycosylation may account for heterogeneity amongst H-2Db molecules. This would explain the presence of the different H-2Db molecules defined here. It follows that differences in glycosylation evidently occur both within the B cell population, since H141-31 binds to only a subset of H-2Db molecules on B cells, and between T and B lymphocytes, since resting T cells do not bind H141-31 MoAb.",
author = "O'Neill, {H C}",
year = "1991",
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language = "English",
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Identification of class I H-2Db molecules primarily expressed by B lymphocytes in murine spleen. / O'Neill, H C.

In: Immunology and Cell Biology, Vol. 69 , No. 2, 04.1991, p. 95-102.

Research output: Contribution to journalArticleResearchpeer-review

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AB - Evidence is presented which supports the phenomenon of heterogeneity amongst H-2Db-encoded Class I molecules. Two monoclonal antibodies (MoAb) called H141-31 and B22-249 were used in these studies. Both bind to the 'private' H-2.2 site of H-2Db-encoded molecules, but the binding of B22-249 is determined by carbohydrate moieties, whereas H141-31 appears to bind to a protein-defined epitope. Some H-2Db molecules, identified by the H141-31 MoAb, are primarily expressed on B lymphocytes and not T lymphocytes in spleen. The number of H-2Db molecules which bind H141-31 on B cells was also found to be three- to four-fold less than the number which bound the B22-249 MoAb. B cells of two mutant strains of mice, B6-C.H-2bm13 and B6-C.H-2bm14 which harbour very few nucleotide changes in the H-2Db gene, also show marked reduction in the binding of both antibodies. This suggests that a single common gene encodes both target molecules and that post-translational modifications such as differential glycosylation may account for heterogeneity amongst H-2Db molecules. This would explain the presence of the different H-2Db molecules defined here. It follows that differences in glycosylation evidently occur both within the B cell population, since H141-31 binds to only a subset of H-2Db molecules on B cells, and between T and B lymphocytes, since resting T cells do not bind H141-31 MoAb.

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