Identification of a novel antigen cross-presenting cell type in spleen

Jonathan K H Tan, Ben J C Quah, Kristin L Griffiths, Pravin Periasamy, Ying-Ying Hey, Helen C O'Neill

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27 Citations (Scopus)
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Abstract

Antigen-presenting cells (APC), like dendritic cells (DC), are essential for T-cell activation, leading to immunity or tolerance. Multiple DC subsets each play a unique role in the immune response. Here, a novel splenic dendritic-like APC has been characterized in mice that has immune function and cell surface phenotype distinct from other, described DC subsets. These were identified as a cell type continuously produced in spleen long-term cultures (LTC) and have an in vivo equivalent cell type in mice, namely 'L-DC'. This study characterizes LTC-DC in terms of marker phenotype and function, and compares them with L-DC and other known splenic DC and myeloid subsets. L-DC display a myeloid dendritic-like phenotype equivalent to LTC-DC as CD11c(lo) CD11b(hi) MHC-II(-) CD8α(-) cells, distinct by high accessibility and endocytic capacity for blood-borne antigen. Both LTC-DC and L-DC have strong antigen cross-presentation ability leading to strong activation of CD8(+) T cells, particularly after exposure to lipopolysaccharide. However, they have weak ability to stimulate CD4(+) T cells in antigen-specific responses. Evidence is presented here for a novel DC type produced by in vitro haematopoiesis which has distinct antigen-presenting potential and reflects a DC subset present also in vivo in spleen.

Original languageEnglish
Pages (from-to)1189-99
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Volume15
Issue number5
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Antigen-Presenting Cells
Dendritic Cells
Spleen
T-Lymphocytes
Phenotype
Antigens
Cross-Priming
Hematopoiesis
Antigen Presentation
Lipopolysaccharides
Immunity

Cite this

Tan, Jonathan K H ; Quah, Ben J C ; Griffiths, Kristin L ; Periasamy, Pravin ; Hey, Ying-Ying ; O'Neill, Helen C. / Identification of a novel antigen cross-presenting cell type in spleen. In: Journal of Cellular and Molecular Medicine. 2011 ; Vol. 15, No. 5. pp. 1189-99.
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Identification of a novel antigen cross-presenting cell type in spleen. / Tan, Jonathan K H; Quah, Ben J C; Griffiths, Kristin L; Periasamy, Pravin; Hey, Ying-Ying; O'Neill, Helen C.

In: Journal of Cellular and Molecular Medicine, Vol. 15, No. 5, 2011, p. 1189-99.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Periasamy, Pravin

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AU - O'Neill, Helen C

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