High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis

Ekua W. Brenu, Kevin J. Ashton, Jana Batovska, Donald R. Staines, Sonya M. Marshall-Gradisnik

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)
19 Downloads (Pure)

Abstract

Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.

Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.

Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.

Original languageEnglish
Article numbere102783
JournalPLoS One
Volume9
Issue number9
DOIs
Publication statusPublished - 19 Sep 2014

Fingerprint

Chronic Fatigue Syndrome
encephalitis
MicroRNAs
microRNA
Throughput
Fatigue of materials
Plasmas
Biomarkers
chronic diseases
biomarkers
Biological Phenomena

Cite this

Brenu, Ekua W. ; Ashton, Kevin J. ; Batovska, Jana ; Staines, Donald R. ; Marshall-Gradisnik, Sonya M. / High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis. In: PLoS One. 2014 ; Vol. 9, No. 9.
@article{5c1d72b406334f32aebef0ab4cbda41a,
title = "High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis",
abstract = "Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.",
author = "Brenu, {Ekua W.} and Ashton, {Kevin J.} and Jana Batovska and Staines, {Donald R.} and Marshall-Gradisnik, {Sonya M.}",
year = "2014",
month = "9",
day = "19",
doi = "10.1371/journal.pone.0102783",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis. / Brenu, Ekua W.; Ashton, Kevin J.; Batovska, Jana; Staines, Donald R.; Marshall-Gradisnik, Sonya M.

In: PLoS One, Vol. 9, No. 9, e102783, 19.09.2014.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis

AU - Brenu, Ekua W.

AU - Ashton, Kevin J.

AU - Batovska, Jana

AU - Staines, Donald R.

AU - Marshall-Gradisnik, Sonya M.

PY - 2014/9/19

Y1 - 2014/9/19

N2 - Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.

AB - Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.

UR - http://www.scopus.com/inward/record.url?scp=84907198122&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0102783

DO - 10.1371/journal.pone.0102783

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e102783

ER -