High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms

Takeo Ejima; Fumihiko Ishida; Hiromichi Murata; Mitsunori Toyoda; Tetsuo Harada; Toshihide Tsuru; Tadashi Hatano; Mihiro Yanagihara; Masaki Yamamoto; Haruo Mizutani

doi:10.1364/OE.18.007203

High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms

Takeo Ejima^*, Fumihiko Ishida, Hiromichi Murata, Mitsunori Toyoda, Tetsuo Harada, Toshihide Tsuru, Tadashi Hatano, Mihiro Yanagihara, Masaki Yamamoto, Haruo Mizutani

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

25 Citations (Scopus)

Abstract

We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 μm² field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.

Original language	English
Pages (from-to)	7203-7209
Number of pages	7
Journal	Optics Express
Volume	18
Issue number	7
DOIs	https://doi.org/10.1364/OE.18.007203
Publication status	Published - 29 Mar 2010
Externally published	Yes

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abstract = "We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 μm2 field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.",

author = "Takeo Ejima and Fumihiko Ishida and Hiromichi Murata and Mitsunori Toyoda and Tetsuo Harada and Toshihide Tsuru and Tadashi Hatano and Mihiro Yanagihara and Masaki Yamamoto and Haruo Mizutani",

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T1 - High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms

AU - Ejima, Takeo

AU - Ishida, Fumihiko

AU - Murata, Hiromichi

AU - Toyoda, Mitsunori

AU - Harada, Tetsuo

AU - Tsuru, Toshihide

AU - Hatano, Tadashi

AU - Yanagihara, Mihiro

AU - Yamamoto, Masaki

AU - Mizutani, Haruo

PY - 2010/3/29

Y1 - 2010/3/29

N2 - We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 μm2 field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.

AB - We present and demonstrate the use of an extreme ultraviolet (EUV) microscope that was developed in-house. Images are acquired using Bragg reflection multilayer optics and a laser-produced plasma light source. The upper-limit spatial resolution of the EUV microscope is 130 nm with a 10 ns exposure time and 250 x 250 μm2 field of view. Resolution is superior to that of visible microscopes with the same size of field of view, and the exposure time is short enough to observe fine structures in-vivo. Observation of the cerebral cortex of a mouse is demonstrated.

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High throughput and wide field of view EUV microscope for blur-free one-shot imaging of living organisms

Abstract

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