Heterogeneity amongst splenic stromal cell lines which support dendritic cell hematopoiesis

Genevieve Despars, Helen C. O'Neill

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Long-term cultures (LTC) producing dendritie cells (DC) have been previously established from spleen. LTC support the development of nonadherent cells comprising small DC progenitors and immature DC. Similarly, the splenic stroma STX3, derived from a LTC which ceased DC production, can support DC development from precursors in overlaid bone marrow. The STX3 stroma is an immortalised mixed population of endothelial cells and elongated spindle-shaped cells, thought to be fibroblasts. The stromal cell components of STX3 have been studied here. A panel of 102 cell lines was established by single-cell sorting. A range of clone morphology, including cobblestone cells and elongated spindle-shaped cells, was reflective of heterogeneity in STX3. However, similar expression levels for the endothelial genes ACVRLII ALKI, COL18A1, and MCAM in 13 splenie stromal cell lines suggested that both cell types had endothelial origin. The hematopoietic support function of stromal clones was tested in coculture assays with a bone marrow cell overlay. Splenic stromal cell lines with different morphology were both supporters and nonsuppor-ters of hematopoiesis, in terms of foei formation or release of suspension cells. Cloning of STX3 led to the isolation of a panel of splenie endothelial cell lines heterogeneous in terms of morphology and hematopoietic support function.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume42
Issue number7
DOIs
Publication statusPublished - 5 Sep 2006
Externally publishedYes

Cite this

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title = "Heterogeneity amongst splenic stromal cell lines which support dendritic cell hematopoiesis",
abstract = "Long-term cultures (LTC) producing dendritie cells (DC) have been previously established from spleen. LTC support the development of nonadherent cells comprising small DC progenitors and immature DC. Similarly, the splenic stroma STX3, derived from a LTC which ceased DC production, can support DC development from precursors in overlaid bone marrow. The STX3 stroma is an immortalised mixed population of endothelial cells and elongated spindle-shaped cells, thought to be fibroblasts. The stromal cell components of STX3 have been studied here. A panel of 102 cell lines was established by single-cell sorting. A range of clone morphology, including cobblestone cells and elongated spindle-shaped cells, was reflective of heterogeneity in STX3. However, similar expression levels for the endothelial genes ACVRLII ALKI, COL18A1, and MCAM in 13 splenie stromal cell lines suggested that both cell types had endothelial origin. The hematopoietic support function of stromal clones was tested in coculture assays with a bone marrow cell overlay. Splenic stromal cell lines with different morphology were both supporters and nonsuppor-ters of hematopoiesis, in terms of foei formation or release of suspension cells. Cloning of STX3 led to the isolation of a panel of splenie endothelial cell lines heterogeneous in terms of morphology and hematopoietic support function.",
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Heterogeneity amongst splenic stromal cell lines which support dendritic cell hematopoiesis. / Despars, Genevieve; O'Neill, Helen C.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 42, No. 7, 05.09.2006, p. 208-215.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Despars, Genevieve

AU - O'Neill, Helen C.

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AB - Long-term cultures (LTC) producing dendritie cells (DC) have been previously established from spleen. LTC support the development of nonadherent cells comprising small DC progenitors and immature DC. Similarly, the splenic stroma STX3, derived from a LTC which ceased DC production, can support DC development from precursors in overlaid bone marrow. The STX3 stroma is an immortalised mixed population of endothelial cells and elongated spindle-shaped cells, thought to be fibroblasts. The stromal cell components of STX3 have been studied here. A panel of 102 cell lines was established by single-cell sorting. A range of clone morphology, including cobblestone cells and elongated spindle-shaped cells, was reflective of heterogeneity in STX3. However, similar expression levels for the endothelial genes ACVRLII ALKI, COL18A1, and MCAM in 13 splenie stromal cell lines suggested that both cell types had endothelial origin. The hematopoietic support function of stromal clones was tested in coculture assays with a bone marrow cell overlay. Splenic stromal cell lines with different morphology were both supporters and nonsuppor-ters of hematopoiesis, in terms of foei formation or release of suspension cells. Cloning of STX3 led to the isolation of a panel of splenie endothelial cell lines heterogeneous in terms of morphology and hematopoietic support function.

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