Healing cells in the dermis and adipose tissues of the adult pig

Henry E. Young, Jason Ionnis Limnios, Frank Lochner, George McCommon, Gypsy F. Black, Julie Coleman, Kristina C Hawkins, Asa C Black Jr.

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Abstract

Stage-specific antigen-4 (SSEA-4) positive cells and carcinoembryonic antigen-cell adhesion molecule-1 (CEACAM-1)
positive cells, indicative of pluripotent stem cells and totipotent stem cells, respectively, have been
isolated and characterized from the skeletal muscle and blood of adult animals, including humans. The current
study was undertaken to determine their location in the dermis and underlying connective tissues of the adult pig.
Adult pigs were euthanized following the guidelines of Fort Valley State University’s IACUC. The skin (epidermis
through hypodermis) was harvested, fixed, cryosectioned, and stained with the two antibodies: SSEA-4 and CEACAM-1.
SSEA-4 positive cells were located preferentially in the reticular dermis of the skin and to some extent
in the underlying hypodermis. In contrast, CEA-CAM-1 positive stem cells were preferentially located within the
hypodermis of the pig skin within the loose fibrous connective tissues surrounding adipose tissue. CEA-CAM-1
positive cells were also located, to a lesser extent, in the dermis as well. These results demonstrate the presence of
native populations of pluripotent stem cells and totipotent stem cells within the dermis, hypodermis, and adipose
tissue of adult pig skin. Studies are ongoing to address the functional significance of these cells in normal injury
and repair
Original languageEnglish
Number of pages5
JournalJournal of Stem Cell Research
Volume1
Issue number2
Publication statusPublished - 18 Sep 2017

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Dermis
Adipose Tissue
Swine
Totipotent Stem Cells
Subcutaneous Tissue
Pluripotent Stem Cells
Skin
Carcinoembryonic Antigen
Cell Adhesion Molecules
Antigens
Connective Tissue
Animal Care Committees
Skeletal Muscle
Stem Cells
Guidelines
Antibodies
Population

Cite this

Young, H. E., Limnios, J. I., Lochner, F., McCommon, G., Black, G. F., Coleman, J., ... Black Jr., A. C. (2017). Healing cells in the dermis and adipose tissues of the adult pig. Journal of Stem Cell Research, 1(2).
Young, Henry E. ; Limnios, Jason Ionnis ; Lochner, Frank ; McCommon, George ; Black, Gypsy F. ; Coleman, Julie ; Hawkins, Kristina C ; Black Jr., Asa C. / Healing cells in the dermis and adipose tissues of the adult pig. In: Journal of Stem Cell Research. 2017 ; Vol. 1, No. 2.
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abstract = "Stage-specific antigen-4 (SSEA-4) positive cells and carcinoembryonic antigen-cell adhesion molecule-1 (CEACAM-1)positive cells, indicative of pluripotent stem cells and totipotent stem cells, respectively, have beenisolated and characterized from the skeletal muscle and blood of adult animals, including humans. The currentstudy was undertaken to determine their location in the dermis and underlying connective tissues of the adult pig.Adult pigs were euthanized following the guidelines of Fort Valley State University’s IACUC. The skin (epidermisthrough hypodermis) was harvested, fixed, cryosectioned, and stained with the two antibodies: SSEA-4 and CEACAM-1.SSEA-4 positive cells were located preferentially in the reticular dermis of the skin and to some extentin the underlying hypodermis. In contrast, CEA-CAM-1 positive stem cells were preferentially located within thehypodermis of the pig skin within the loose fibrous connective tissues surrounding adipose tissue. CEA-CAM-1positive cells were also located, to a lesser extent, in the dermis as well. These results demonstrate the presence ofnative populations of pluripotent stem cells and totipotent stem cells within the dermis, hypodermis, and adiposetissue of adult pig skin. Studies are ongoing to address the functional significance of these cells in normal injuryand repair",
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Young, HE, Limnios, JI, Lochner, F, McCommon, G, Black, GF, Coleman, J, Hawkins, KC & Black Jr., AC 2017, 'Healing cells in the dermis and adipose tissues of the adult pig' Journal of Stem Cell Research, vol. 1, no. 2.

Healing cells in the dermis and adipose tissues of the adult pig. / Young, Henry E.; Limnios, Jason Ionnis; Lochner, Frank; McCommon, George; Black, Gypsy F.; Coleman, Julie; Hawkins, Kristina C; Black Jr., Asa C.

In: Journal of Stem Cell Research, Vol. 1, No. 2, 18.09.2017.

Research output: Contribution to journalArticleResearchpeer-review

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Young HE, Limnios JI, Lochner F, McCommon G, Black GF, Coleman J et al. Healing cells in the dermis and adipose tissues of the adult pig. Journal of Stem Cell Research. 2017 Sep 18;1(2).