Growth hormone receptors and binding proteins

Michael J. Waters; Steven A. Spencer; D Leung; R. G. Hammonds; G Cachianes; William J. Henzel; W I Wood; R. T. Barnard; Patrick Quirk; Gary P Hamlin

doi:10.1016/B978-0-407-01473-2.50006-0

Growth hormone receptors and binding proteins

Michael J. Waters, Steven A. Spencer, D Leung, R. G. Hammonds, G Cachianes, William J. Henzel, W I Wood, R. T. Barnard, Patrick Quirk, Gary P Hamlin

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

Abstract

Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.

Original language	English
Title of host publication	Biotechnology in Growth Regulation
Editors	R. B. Heap, C. G. Prosser, G. E. Lamming
Place of Publication	London
Publisher	Butterworths
Pages	15-25
Number of pages	11
ISBN (Print)	978-0-407-01473-2, 040701473X
DOIs	https://doi.org/10.1016/B978-0-407-01473-2.50006-0
Publication status	Published - 1989
Externally published	Yes

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title = "Growth hormone receptors and binding proteins",

abstract = "Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.",

author = "Waters, {Michael J.} and Spencer, {Steven A.} and D Leung and Hammonds, {R. G.} and G Cachianes and Henzel, {William J.} and Wood, {W I} and Barnard, {R. T.} and Patrick Quirk and Hamlin, {Gary P}",

year = "1989",

doi = "10.1016/B978-0-407-01473-2.50006-0",

language = "English",

isbn = " 978-0-407-01473-2",

pages = "15--25",

editor = "Heap, {R. B. } and Prosser, {C. G.} and Lamming, {G. E. }",

booktitle = "Biotechnology in Growth Regulation",

publisher = "Butterworths",

}

TY - CHAP

T1 - Growth hormone receptors and binding proteins

AU - Waters, Michael J.

AU - Spencer, Steven A.

AU - Leung, D

AU - Hammonds, R. G.

AU - Cachianes, G

AU - Henzel, William J.

AU - Wood, W I

AU - Barnard, R. T.

AU - Quirk, Patrick

AU - Hamlin, Gary P

PY - 1989

Y1 - 1989

N2 - Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.

AB - Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.

U2 - 10.1016/B978-0-407-01473-2.50006-0

DO - 10.1016/B978-0-407-01473-2.50006-0

M3 - Chapter

SN - 978-0-407-01473-2

SN - 040701473X

SP - 15

EP - 25

BT - Biotechnology in Growth Regulation

A2 - Heap, R. B.

A2 - Prosser, C. G.

A2 - Lamming, G. E.

PB - Butterworths

CY - London

ER -

Growth hormone receptors and binding proteins

Abstract

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