Growth hormone receptors and binding proteins

Michael J. Waters, Steven A. Spencer, D Leung, R. G. Hammonds, G Cachianes, William J. Henzel, W I Wood, R. T. Barnard, Patrick Quirk, Gary P Hamlin

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.
Original languageEnglish
Title of host publicationBiotechnology in Growth Regulation
EditorsR. B. Heap, C. G. Prosser, G. E. Lamming
Place of PublicationLondon
PublisherButterworths
Pages15-25
Number of pages11
ISBN (Print) 978-0-407-01473-2, 040701473X
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Somatotropin Receptors
Rabbits
Liver
Carrier Proteins
Prolactin Receptors
COS Cells
Human Mammary Glands
Lactation
Growth Hormone
Sequence Analysis
somatotropin-binding protein
Organism Cloning
Blood Proteins
Epitopes
Monoclonal Antibodies
Hormones
Growth
Serum

Cite this

Waters, M. J., Spencer, S. A., Leung, D., Hammonds, R. G., Cachianes, G., Henzel, W. J., ... Hamlin, G. P. (1989). Growth hormone receptors and binding proteins. In R. B. Heap, C. G. Prosser, & G. E. Lamming (Eds.), Biotechnology in Growth Regulation (pp. 15-25). London : Butterworths. https://doi.org/10.1016/B978-0-407-01473-2.50006-0
Waters, Michael J. ; Spencer, Steven A. ; Leung, D ; Hammonds, R. G. ; Cachianes, G ; Henzel, William J. ; Wood, W I ; Barnard, R. T. ; Quirk, Patrick ; Hamlin, Gary P. / Growth hormone receptors and binding proteins. Biotechnology in Growth Regulation. editor / R. B. Heap ; C. G. Prosser ; G. E. Lamming. London : Butterworths, 1989. pp. 15-25
@inbook{4c6c90c35000487584e9d88b67a80047,
title = "Growth hormone receptors and binding proteins",
abstract = "Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34{\%} homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.",
author = "Waters, {Michael J.} and Spencer, {Steven A.} and D Leung and Hammonds, {R. G.} and G Cachianes and Henzel, {William J.} and Wood, {W I} and Barnard, {R. T.} and Patrick Quirk and Hamlin, {Gary P}",
year = "1989",
doi = "10.1016/B978-0-407-01473-2.50006-0",
language = "English",
isbn = "978-0-407-01473-2",
pages = "15--25",
editor = "Heap, {R. B. } and Prosser, {C. G.} and Lamming, {G. E. }",
booktitle = "Biotechnology in Growth Regulation",
publisher = "Butterworths",

}

Waters, MJ, Spencer, SA, Leung, D, Hammonds, RG, Cachianes, G, Henzel, WJ, Wood, WI, Barnard, RT, Quirk, P & Hamlin, GP 1989, Growth hormone receptors and binding proteins. in RB Heap, CG Prosser & GE Lamming (eds), Biotechnology in Growth Regulation. Butterworths, London , pp. 15-25. https://doi.org/10.1016/B978-0-407-01473-2.50006-0

Growth hormone receptors and binding proteins. / Waters, Michael J.; Spencer, Steven A.; Leung, D; Hammonds, R. G.; Cachianes, G; Henzel, William J.; Wood, W I; Barnard, R. T.; Quirk, Patrick; Hamlin, Gary P.

Biotechnology in Growth Regulation. ed. / R. B. Heap; C. G. Prosser; G. E. Lamming. London : Butterworths, 1989. p. 15-25.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

TY - CHAP

T1 - Growth hormone receptors and binding proteins

AU - Waters, Michael J.

AU - Spencer, Steven A.

AU - Leung, D

AU - Hammonds, R. G.

AU - Cachianes, G

AU - Henzel, William J.

AU - Wood, W I

AU - Barnard, R. T.

AU - Quirk, Patrick

AU - Hamlin, Gary P

PY - 1989

Y1 - 1989

N2 - Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.

AB - Growth hormone receptors are found in a wide variety of tissues and are thought to mediate the various actions of growth hormone. Recently, a growth hormone binding protein was demonstrated in serum and shown to have antigenic identity with the liver growth hormone receptor by use of a panel of monoclonal antibodies to the receptor. Here we describe the purification, part sequence and cloning of the rabbit liver growth hormone receptor. Purification and N terminal sequence analysis of the rabbit serum binding protein for GH showed it to be identical to the extracellular region of the rabbit liver GH receptor. Rabbit liver receptor and human binding protein sequences were expressed in COS-7 cells and shown to display predicted hormone specificity and antigenic characteristics. Finally, the rabbit mammary gland prolactin receptor was purified and part sequenced. This showed 34% homology with the rabbit liver GH receptor, and therefore constitutes the second member of a new class of transmembrane receptors regulating growth and lactation.

U2 - 10.1016/B978-0-407-01473-2.50006-0

DO - 10.1016/B978-0-407-01473-2.50006-0

M3 - Chapter

SN - 978-0-407-01473-2

SN - 040701473X

SP - 15

EP - 25

BT - Biotechnology in Growth Regulation

A2 - Heap, R. B.

A2 - Prosser, C. G.

A2 - Lamming, G. E.

PB - Butterworths

CY - London

ER -

Waters MJ, Spencer SA, Leung D, Hammonds RG, Cachianes G, Henzel WJ et al. Growth hormone receptors and binding proteins. In Heap RB, Prosser CG, Lamming GE, editors, Biotechnology in Growth Regulation. London : Butterworths. 1989. p. 15-25 https://doi.org/10.1016/B978-0-407-01473-2.50006-0