Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels

Rajshekhar Chatterjee; Jyotsna Batra; Sudipta Das; Surendra Kumar Sharma; Balaram Ghosh

doi:10.1016/j.jaci.2008.04.030

Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels

Rajshekhar Chatterjee, Jyotsna Batra, Sudipta Das, Surendra Kumar Sharma, Balaram Ghosh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

51 Citations (Scopus)

Abstract

Background:

In view of the hygiene hypothesis and the involvement of acidic mammalian chitinase (CHIA) in the effector responses of IL-13 with asthma, CHIA (GeneID-27159) is a potential asthma candidate gene.

Objective:

To investigate the association of CHIA polymorphisms with atopic asthma and serum total IgE levels.

Methods:

Twenty-one single nucleotide polymorphisms were identified by sequencing DNA of 60 individuals. On the basis of linkage disequilibrium, 6 polymorphisms were selected and genotyped in unrelated atopic patients with asthma (N = 270) and controls (N = 292) and an independent pediatric cohort (patients, 150; controls, 101). Electrophoretic mobility shift assay and reporter gene assays were also performed.

Results:

The rs3806448G/A promoter polymorphism showed significant association with atopic asthma (P_adult = .00001 and P_pediatric = .0002) and serum total IgE (P < .05). Also rs2282290G/A was associated with atopic asthma (P_adult = .00009 and P_pediatric = .00003), whereas the rs10494132C/T polymorphism was associated with serum total IgE in the patients (P < .05). We also showed that the promoter single nucleotide polymorphisms altered the transcriptional activity of CHIA promoter and the C to T substitution at rs10494132 abrogated the Octamer transcription factor-1 (Oct-1) binding site.

Conclusion:

Our results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population.

Original language	English
Pages (from-to)	202-208.e7
Number of pages	14
Journal	Journal of Allergy and Clinical Immunology
Volume	122
Issue number	1
DOIs	https://doi.org/10.1016/j.jaci.2008.04.030
Publication status	Published - Jul 2008
Externally published	Yes

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10.1016/j.jaci.2008.04.030

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title = "Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels",

abstract = "Background: In view of the hygiene hypothesis and the involvement of acidic mammalian chitinase (CHIA) in the effector responses of IL-13 with asthma, CHIA (GeneID-27159) is a potential asthma candidate gene. Objective: To investigate the association of CHIA polymorphisms with atopic asthma and serum total IgE levels. Methods: Twenty-one single nucleotide polymorphisms were identified by sequencing DNA of 60 individuals. On the basis of linkage disequilibrium, 6 polymorphisms were selected and genotyped in unrelated atopic patients with asthma (N = 270) and controls (N = 292) and an independent pediatric cohort (patients, 150; controls, 101). Electrophoretic mobility shift assay and reporter gene assays were also performed. Results: The rs3806448G/A promoter polymorphism showed significant association with atopic asthma (Padult = .00001 and Ppediatric = .0002) and serum total IgE (P < .05). Also rs2282290G/A was associated with atopic asthma (Padult = .00009 and Ppediatric = .00003), whereas the rs10494132C/T polymorphism was associated with serum total IgE in the patients (P < .05). We also showed that the promoter single nucleotide polymorphisms altered the transcriptional activity of CHIA promoter and the C to T substitution at rs10494132 abrogated the Octamer transcription factor-1 (Oct-1) binding site. Conclusion: Our results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population.",

author = "Rajshekhar Chatterjee and Jyotsna Batra and Sudipta Das and Sharma, {Surendra Kumar} and Balaram Ghosh",

year = "2008",

month = jul,

doi = "10.1016/j.jaci.2008.04.030",

language = "English",

volume = "122",

pages = "202--208.e7",

journal = "Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

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}

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T1 - Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels

AU - Chatterjee, Rajshekhar

AU - Batra, Jyotsna

AU - Das, Sudipta

AU - Sharma, Surendra Kumar

AU - Ghosh, Balaram

PY - 2008/7

Y1 - 2008/7

N2 - Background: In view of the hygiene hypothesis and the involvement of acidic mammalian chitinase (CHIA) in the effector responses of IL-13 with asthma, CHIA (GeneID-27159) is a potential asthma candidate gene. Objective: To investigate the association of CHIA polymorphisms with atopic asthma and serum total IgE levels. Methods: Twenty-one single nucleotide polymorphisms were identified by sequencing DNA of 60 individuals. On the basis of linkage disequilibrium, 6 polymorphisms were selected and genotyped in unrelated atopic patients with asthma (N = 270) and controls (N = 292) and an independent pediatric cohort (patients, 150; controls, 101). Electrophoretic mobility shift assay and reporter gene assays were also performed. Results: The rs3806448G/A promoter polymorphism showed significant association with atopic asthma (Padult = .00001 and Ppediatric = .0002) and serum total IgE (P < .05). Also rs2282290G/A was associated with atopic asthma (Padult = .00009 and Ppediatric = .00003), whereas the rs10494132C/T polymorphism was associated with serum total IgE in the patients (P < .05). We also showed that the promoter single nucleotide polymorphisms altered the transcriptional activity of CHIA promoter and the C to T substitution at rs10494132 abrogated the Octamer transcription factor-1 (Oct-1) binding site. Conclusion: Our results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population.

AB - Background: In view of the hygiene hypothesis and the involvement of acidic mammalian chitinase (CHIA) in the effector responses of IL-13 with asthma, CHIA (GeneID-27159) is a potential asthma candidate gene. Objective: To investigate the association of CHIA polymorphisms with atopic asthma and serum total IgE levels. Methods: Twenty-one single nucleotide polymorphisms were identified by sequencing DNA of 60 individuals. On the basis of linkage disequilibrium, 6 polymorphisms were selected and genotyped in unrelated atopic patients with asthma (N = 270) and controls (N = 292) and an independent pediatric cohort (patients, 150; controls, 101). Electrophoretic mobility shift assay and reporter gene assays were also performed. Results: The rs3806448G/A promoter polymorphism showed significant association with atopic asthma (Padult = .00001 and Ppediatric = .0002) and serum total IgE (P < .05). Also rs2282290G/A was associated with atopic asthma (Padult = .00009 and Ppediatric = .00003), whereas the rs10494132C/T polymorphism was associated with serum total IgE in the patients (P < .05). We also showed that the promoter single nucleotide polymorphisms altered the transcriptional activity of CHIA promoter and the C to T substitution at rs10494132 abrogated the Octamer transcription factor-1 (Oct-1) binding site. Conclusion: Our results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population.

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Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels

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