Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors

Janice L Abbey, Holger Karsunky, Thomas Serwold, Peter Papathanasiou, Irving L Weissman, Helen C O'Neill

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Abstract

Germline transcription has been described for both immunoglobulin and T-cell receptor (TCR) genes, raising questions of their functional significance during haematopoiesis. Previously, an immature murine T-cell line was shown to bind antibody to TCR-Vβ8.2 in absence of anti-Cβ antibody binding, and an equivalent cell subset was also identified in the mesenteric lymph node. Here, we investigate whether germline transcription and cell surface Vβ8.2 expression could therefore represent a potential marker of T-cell progenitors. Cells with the TCR phenotype of Vβ8.2(+) Cβ(-) are found in several lymphoid sites, and among the lineage-negative (Lin(-)) fraction of hematopoietic progenitors in bone marrow (BM). Cell surface marker analysis of these cells identified subsets reflecting common lymphoid progenitors, common myeloid progenitors and multipotential progenitors. To assess whether the Lin(-) Vβ8.2(+) Cβ(-) BM subset contains hematopoietic progenitors, cells were sorted and adoptively transferred into sub-lethally irradiated recipients. No T-cell or myeloid progeny were detected following introduction of cells via the intrathymic or intravenous routes. However, B-cell development was detected in spleen. This pattern of restricted in vivo reconstitution disputes Lin(-) Vβ8.2(+) Cβ(-) BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development.

Original languageEnglish
Pages (from-to)1956-1964
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 2015
Externally publishedYes

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T-Cell Antigen Receptor
Bone Marrow Cells
T-Lymphocytes
B-Lymphocytes
Lymphoid Progenitor Cells
Bone Marrow
Myeloid Progenitor Cells
T-Cell Receptor Genes
Dissent and Disputes
Hematopoiesis
Hematopoietic Stem Cells
peptide V
Immunoglobulins
Anti-Idiotypic Antibodies
Spleen
Lymph Nodes
Phenotype
Cell Line
Antibodies

Cite this

Abbey, Janice L ; Karsunky, Holger ; Serwold, Thomas ; Papathanasiou, Peter ; Weissman, Irving L ; O'Neill, Helen C. / Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors. In: Journal of Cellular and Molecular Medicine. 2015 ; Vol. 19, No. 8. pp. 1956-1964.
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abstract = "Germline transcription has been described for both immunoglobulin and T-cell receptor (TCR) genes, raising questions of their functional significance during haematopoiesis. Previously, an immature murine T-cell line was shown to bind antibody to TCR-Vβ8.2 in absence of anti-Cβ antibody binding, and an equivalent cell subset was also identified in the mesenteric lymph node. Here, we investigate whether germline transcription and cell surface Vβ8.2 expression could therefore represent a potential marker of T-cell progenitors. Cells with the TCR phenotype of Vβ8.2(+) Cβ(-) are found in several lymphoid sites, and among the lineage-negative (Lin(-)) fraction of hematopoietic progenitors in bone marrow (BM). Cell surface marker analysis of these cells identified subsets reflecting common lymphoid progenitors, common myeloid progenitors and multipotential progenitors. To assess whether the Lin(-) Vβ8.2(+) Cβ(-) BM subset contains hematopoietic progenitors, cells were sorted and adoptively transferred into sub-lethally irradiated recipients. No T-cell or myeloid progeny were detected following introduction of cells via the intrathymic or intravenous routes. However, B-cell development was detected in spleen. This pattern of restricted in vivo reconstitution disputes Lin(-) Vβ8.2(+) Cβ(-) BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development.",
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Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors. / Abbey, Janice L; Karsunky, Holger; Serwold, Thomas; Papathanasiou, Peter; Weissman, Irving L; O'Neill, Helen C.

In: Journal of Cellular and Molecular Medicine, Vol. 19, No. 8, 08.2015, p. 1956-1964.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Abbey, Janice L

AU - Karsunky, Holger

AU - Serwold, Thomas

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AU - Weissman, Irving L

AU - O'Neill, Helen C

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AB - Germline transcription has been described for both immunoglobulin and T-cell receptor (TCR) genes, raising questions of their functional significance during haematopoiesis. Previously, an immature murine T-cell line was shown to bind antibody to TCR-Vβ8.2 in absence of anti-Cβ antibody binding, and an equivalent cell subset was also identified in the mesenteric lymph node. Here, we investigate whether germline transcription and cell surface Vβ8.2 expression could therefore represent a potential marker of T-cell progenitors. Cells with the TCR phenotype of Vβ8.2(+) Cβ(-) are found in several lymphoid sites, and among the lineage-negative (Lin(-)) fraction of hematopoietic progenitors in bone marrow (BM). Cell surface marker analysis of these cells identified subsets reflecting common lymphoid progenitors, common myeloid progenitors and multipotential progenitors. To assess whether the Lin(-) Vβ8.2(+) Cβ(-) BM subset contains hematopoietic progenitors, cells were sorted and adoptively transferred into sub-lethally irradiated recipients. No T-cell or myeloid progeny were detected following introduction of cells via the intrathymic or intravenous routes. However, B-cell development was detected in spleen. This pattern of restricted in vivo reconstitution disputes Lin(-) Vβ8.2(+) Cβ(-) BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development.

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