Expansion of hMSCs and Their Application

Zhidao Xia, Joanna Bishop

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

1 Citation (Scopus)


Human mesenchymal stem cells (hMSCs) can generate bone, cartilage, tendon, muscle, ligament, fat, and are able to differentiate to cardiomyocytes, neurons and astrocytes, etc. in defined conditions. They not only differentiate to the cell type required for local tissue regeneration, but also secrete a large number of bioactive molecules following implantation that ultimately lead to reformation of tissues at the site of injury. The hMSCs can be isolated with consent from bone marrow, adipose tissue, dental pulp, umbilical cord blood and matrix, and potentially peripheral blood. They have been widely used for clinical application without the ethical issues implicated with embryonic stem cells. However, they are a rare population in bone marrow and other tissues. In order to reach the number required for clinical use, they need to be expanded significantly in vitro. A variety of biotechnologies are applied for hMSC expansion, including static culture by using tissue culture flasks and cell factory and gas-permeable blood bags; or dynamic culture by using spinner flasks, stirred or rotary bioreactors with/without three-dimensional cell carriers. Routine quality controls are essential to avoid contamination, ensure stable phenotype, and monitor cell mutation before clinical applications.
Original languageEnglish
Title of host publicationComprehensive Biotechnology
Number of pages11
ISBN (Electronic)9780080885049
ISBN (Print)9780444533524
Publication statusPublished - 9 Sept 2011
Externally publishedYes


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