Evaluation of 13 rapidly mutating Y-STRs on a Dravidian pedigree

S. Iyavoo*, R. Alghafri, R. Almheiri, T. Haizel

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The effectiveness of a rapidly mutating Y-STRs multiplex assay (RM Y-STRs) containing 13 RM Y-STR markers was compared with the AmpFℓSTR™ Yfiler™ PCR Amplification Kit (Yfiler) with conventional Y-STR markers for their efficiency in differentiating males within the same paternal lineage. Samples from 4 generations comprising 16 Dravidian males (15 father-son pairs) were analysed with both assays. Mutations were observed in 3 father-son pairs in the RM Y-STRs profiles while only 2 mutations were observed in the Y-filer profiles. Even though not many mutations were observed as anticipated, this study still emphasised the importance of RM Y-STRs when differentiation between males within the same paternal lineage is required and also indicated the need for mutation rates for different populations.

Original languageEnglish
JournalForensic Science International: Genetics Supplement Series
DOIs
Publication statusE-pub ahead of print - 26 Sep 2019
Externally publishedYes

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Pedigree
Nuclear Family
Fathers
Mutation
Mutation Rate
Polymerase Chain Reaction
Population

Cite this

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title = "Evaluation of 13 rapidly mutating Y-STRs on a Dravidian pedigree",
abstract = "The effectiveness of a rapidly mutating Y-STRs multiplex assay (RM Y-STRs) containing 13 RM Y-STR markers was compared with the AmpFℓSTR™ Yfiler™ PCR Amplification Kit (Yfiler) with conventional Y-STR markers for their efficiency in differentiating males within the same paternal lineage. Samples from 4 generations comprising 16 Dravidian males (15 father-son pairs) were analysed with both assays. Mutations were observed in 3 father-son pairs in the RM Y-STRs profiles while only 2 mutations were observed in the Y-filer profiles. Even though not many mutations were observed as anticipated, this study still emphasised the importance of RM Y-STRs when differentiation between males within the same paternal lineage is required and also indicated the need for mutation rates for different populations.",
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Evaluation of 13 rapidly mutating Y-STRs on a Dravidian pedigree. / Iyavoo, S.; Alghafri, R.; Almheiri, R.; Haizel, T.

In: Forensic Science International: Genetics Supplement Series, 26.09.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Alghafri, R.

AU - Almheiri, R.

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PY - 2019/9/26

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