ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells

Amanda Forbes, Andrew K. Davey, Anthony V. Perkins, Gary D. Grant, Amelia J. McFarland, Catherine M. McDermott, Shailendra Anoopkumar-Dukie

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4 Citations (Scopus)

Abstract

Pyocyanin (PCN), a virulence factor produced by Pseudomonas aeruginosa, has many damaging effects on mammalian cells. Several lines of evidence suggest that this damage is primarily mediated by its ability to generate oxidative stress. However mechanisms underlying PCN-induced oxidative injury remain unclear. Although oxidative stress and subsequent MAPK signaling has been shown to modulate cell death in other models, its role in PCN-induced cytotoxicity remains unknown. Therefore the aim of this study was to investigate the role of redox-sensitive MAPK in PCN-induced toxicity in A549 cells. Here we show that PCN (50 μM) rapidly increased ERK1/2 phosphorylation after 5 min. Pre-treatment of A549 cells with the MEK1/2 inhibitor U0126 (10 μM) decreased PCN-induced ERK1/2 phosphorylation and protected cells against apoptosis and cell injury suggesting a role for ERK signalling. In contrast, JNK and p38 MAPK phosphorylation remained unchanged following exposure to PCN and pretreatment with either the JNK or p38 MAPK inhibitors (10 μM SP600125 and 10 μM SB203580, respectively) did not afford protection against PCN toxicity. This would suggest that PCN-induced cytotoxicity appears to occur independently of JNK and p38 MAPK signaling pathways. Finally, although we confirm that oxidative stress contributes to PCN-induced toxicity, our data suggest the contribution of oxidative stress is independent of ERK1/2 signaling. These findings may provide insight for novel targeted therapies to reduce PCN-mediated lung injury in patients with chronic P. aeruginosa respiratory infections.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalChemico-Biological Interactions
Volume208
DOIs
Publication statusPublished - 5 Feb 2014

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Pyocyanine
Toxicity
Epithelial Cells
Chemical activation
Oxidative stress
Phosphorylation
Oxidative Stress
p38 Mitogen-Activated Protein Kinases
Cytotoxicity
Pseudomonas aeruginosa
Wounds and Injuries
Lung Injury
Virulence Factors
Cell death
Respiratory Tract Infections
Oxidation-Reduction

Cite this

Forbes, A., Davey, A. K., Perkins, A. V., Grant, G. D., McFarland, A. J., McDermott, C. M., & Anoopkumar-Dukie, S. (2014). ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells. Chemico-Biological Interactions, 208, 58-63. https://doi.org/10.1016/j.cbi.2013.11.016
Forbes, Amanda ; Davey, Andrew K. ; Perkins, Anthony V. ; Grant, Gary D. ; McFarland, Amelia J. ; McDermott, Catherine M. ; Anoopkumar-Dukie, Shailendra. / ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells. In: Chemico-Biological Interactions. 2014 ; Vol. 208. pp. 58-63.
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ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells. / Forbes, Amanda; Davey, Andrew K.; Perkins, Anthony V.; Grant, Gary D.; McFarland, Amelia J.; McDermott, Catherine M.; Anoopkumar-Dukie, Shailendra.

In: Chemico-Biological Interactions, Vol. 208, 05.02.2014, p. 58-63.

Research output: Contribution to journalArticleResearchpeer-review

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