Effects of dietary selenium on post-ischemic expression of antioxidant mRNA

Kylie Venardos, Kevin Ashton, John Headrick, Anthony Perkins

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume270
Issue number1-2
DOIs
Publication statusPublished - Feb 2005
Externally publishedYes

Fingerprint

Selenium
phospholipid-hydroperoxide glutathione peroxidase
Thioredoxin Reductase 2
Reperfusion
Antioxidants
Thioredoxin Reductase 1
Ischemia
Messenger RNA
Thioredoxins
Peroxiredoxins
Oxidative stress
Rats
Oxidative Stress
Thioredoxin-Disulfide Reductase
Recovery
Glutathione Reductase
Transcription
Glutathione Peroxidase
Gene expression
Glutathione

Cite this

Venardos, Kylie ; Ashton, Kevin ; Headrick, John ; Perkins, Anthony. / Effects of dietary selenium on post-ischemic expression of antioxidant mRNA. In: Molecular and Cellular Biochemistry. 2005 ; Vol. 270, No. 1-2. pp. 131-138.
@article{d2d9fbb6560d40919a90e0f5ae223560,
title = "Effects of dietary selenium on post-ischemic expression of antioxidant mRNA",
abstract = "Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion.",
author = "Kylie Venardos and Kevin Ashton and John Headrick and Anthony Perkins",
year = "2005",
month = "2",
doi = "10.1007/s11010-005-5279-y",
language = "English",
volume = "270",
pages = "131--138",
journal = "Enzymologia",
issn = "0013-9424",
publisher = "Springer",
number = "1-2",

}

Effects of dietary selenium on post-ischemic expression of antioxidant mRNA. / Venardos, Kylie; Ashton, Kevin; Headrick, John; Perkins, Anthony.

In: Molecular and Cellular Biochemistry, Vol. 270, No. 1-2, 02.2005, p. 131-138.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effects of dietary selenium on post-ischemic expression of antioxidant mRNA

AU - Venardos, Kylie

AU - Ashton, Kevin

AU - Headrick, John

AU - Perkins, Anthony

PY - 2005/2

Y1 - 2005/2

N2 - Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion.

AB - Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion.

UR - http://www.scopus.com/inward/record.url?scp=15844415950&partnerID=8YFLogxK

U2 - 10.1007/s11010-005-5279-y

DO - 10.1007/s11010-005-5279-y

M3 - Article

VL - 270

SP - 131

EP - 138

JO - Enzymologia

JF - Enzymologia

SN - 0013-9424

IS - 1-2

ER -