Effects of aflatoxin B1 on in vitro cultures of Nicotiana tabacum var. Samsun - 2: Root and shoot development in tobacco plantlets

M. McLean; M. P. Watt; P. Berjak; M. F. Dutton; C. Snyman

doi:10.1007/BF01371099

Effects of aflatoxin B₁ on in vitro cultures of Nicotiana tabacum var. Samsun - 2: Root and shoot development in tobacco plantlets

M. McLean^*, M. P. Watt, P. Berjak, M. F. Dutton, C. Snyman

^*Corresponding author for this work

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Abstract

The effects of aflatoxin B₁, (0.5-25 μg ml^-1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 μg ml^-1 AFB₁, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB₁ concentration. The number of explants developing roots was reduced to 34% at the highest (25 μg ml^-1) AFB₁ concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB₁ by the plantlets. Using immunocytochemical techniques, AFB₁ was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.

Original language	English
Pages (from-to)	107-117
Number of pages	11
Journal	Mycopathologia
Volume	125
Issue number	2
DOIs	https://doi.org/10.1007/BF01371099
Publication status	Published - 1 Feb 1994
Externally published	Yes

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title = "Effects of aflatoxin B1 on in vitro cultures of Nicotiana tabacum var. Samsun - 2: Root and shoot development in tobacco plantlets",

abstract = "The effects of aflatoxin B1, (0.5-25 μg ml-1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 μg ml-1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 μg ml-1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.",

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AU - McLean, M.

AU - Watt, M. P.

AU - Berjak, P.

AU - Dutton, M. F.

AU - Snyman, C.

PY - 1994/2/1

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N2 - The effects of aflatoxin B1, (0.5-25 μg ml-1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 μg ml-1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 μg ml-1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.

AB - The effects of aflatoxin B1, (0.5-25 μg ml-1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 μg ml-1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 μg ml-1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.

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Effects of aflatoxin B₁ on in vitro cultures of Nicotiana tabacum var. Samsun - 2: Root and shoot development in tobacco plantlets

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