Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle

Vernon G. Coffey, Donald W. Reeder, Graeme I. Lancaster, Wee Kian Yeo, Mark A. Febbraio, Ben B. Yaspelkis, John A. Hawley

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Abstract

PURPOSE: Regulation of skeletal muscle mass is highly dependent on contractile loading. The purpose of this study was to examine changes in growth factor and inflammatory pathways following high-frequency resistance training. METHODS: Using a novel design in which male Sprague-Dawley rats undertook a "stacked" resistance training protocol designed to generate a summation of transient exercise-induced signaling responses (four bouts of three sets × 10 repetitions of squat exercise, separated by 3 h of recovery), we determined the effects of high training frequency on signaling pathways and transcriptional activity regulating muscle mass. RESULTS: The stacked training regimen resulted in acute suppression of insulin-like growth factor 1 mRNA abundance (P < 0.05) and Akt phosphorylation (P < 0.05), an effect that persisted 48 h after the final training bout. Conversely, stacked training elicited a coordinated increase in the expression of tumor necrosis factor alpha, inhibitor kappa B kinase alpha/beta activity (P < 0.05), and p38 mitogen-activated protein kinase phosphorylation (P < 0.05) at 3 h after each training bout. In addition, the stacked series of resistance exercise bouts induced an increase in p70 S6 kinase phosphorylation 3 h after bouts ×3 and ×4, independent of the phosphorylation state of Akt. CONCLUSIONS: Our results indicate that high resistance training frequency extends the transient activation of inflammatory signaling cascades, concomitant with persistent suppression of key mediators of anabolic responses. We provide novel insights into the effects of the timing of exercise-induced overload and recovery on signal transduction pathways and transcriptional activity regulating skeletal muscle mass in vivo.

Original languageEnglish
Pages (from-to)2135-2144
Number of pages10
JournalMedicine and Science in Sports and Exercise
Volume39
Issue number12
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

Fingerprint

Resistance Training
Skeletal Muscle
Phosphorylation
70-kDa Ribosomal Protein S6 Kinases
Somatomedins
p38 Mitogen-Activated Protein Kinases
Sprague Dawley Rats
Signal Transduction
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Tumor Necrosis Factor-alpha
Muscles
Messenger RNA

Cite this

Coffey, V. G., Reeder, D. W., Lancaster, G. I., Yeo, W. K., Febbraio, M. A., Yaspelkis, B. B., & Hawley, J. A. (2007). Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle. Medicine and Science in Sports and Exercise, 39(12), 2135-2144. https://doi.org/10.1249/mss.0b013e31815729b6
Coffey, Vernon G. ; Reeder, Donald W. ; Lancaster, Graeme I. ; Yeo, Wee Kian ; Febbraio, Mark A. ; Yaspelkis, Ben B. ; Hawley, John A. / Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle. In: Medicine and Science in Sports and Exercise. 2007 ; Vol. 39, No. 12. pp. 2135-2144.
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Coffey, VG, Reeder, DW, Lancaster, GI, Yeo, WK, Febbraio, MA, Yaspelkis, BB & Hawley, JA 2007, 'Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle' Medicine and Science in Sports and Exercise, vol. 39, no. 12, pp. 2135-2144. https://doi.org/10.1249/mss.0b013e31815729b6

Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle. / Coffey, Vernon G.; Reeder, Donald W.; Lancaster, Graeme I.; Yeo, Wee Kian; Febbraio, Mark A.; Yaspelkis, Ben B.; Hawley, John A.

In: Medicine and Science in Sports and Exercise, Vol. 39, No. 12, 12.2007, p. 2135-2144.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Effect of high-frequency resistance exercise on adaptive responses in skeletal muscle

AU - Coffey, Vernon G.

AU - Reeder, Donald W.

AU - Lancaster, Graeme I.

AU - Yeo, Wee Kian

AU - Febbraio, Mark A.

AU - Yaspelkis, Ben B.

AU - Hawley, John A.

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N2 - PURPOSE: Regulation of skeletal muscle mass is highly dependent on contractile loading. The purpose of this study was to examine changes in growth factor and inflammatory pathways following high-frequency resistance training. METHODS: Using a novel design in which male Sprague-Dawley rats undertook a "stacked" resistance training protocol designed to generate a summation of transient exercise-induced signaling responses (four bouts of three sets × 10 repetitions of squat exercise, separated by 3 h of recovery), we determined the effects of high training frequency on signaling pathways and transcriptional activity regulating muscle mass. RESULTS: The stacked training regimen resulted in acute suppression of insulin-like growth factor 1 mRNA abundance (P < 0.05) and Akt phosphorylation (P < 0.05), an effect that persisted 48 h after the final training bout. Conversely, stacked training elicited a coordinated increase in the expression of tumor necrosis factor alpha, inhibitor kappa B kinase alpha/beta activity (P < 0.05), and p38 mitogen-activated protein kinase phosphorylation (P < 0.05) at 3 h after each training bout. In addition, the stacked series of resistance exercise bouts induced an increase in p70 S6 kinase phosphorylation 3 h after bouts ×3 and ×4, independent of the phosphorylation state of Akt. CONCLUSIONS: Our results indicate that high resistance training frequency extends the transient activation of inflammatory signaling cascades, concomitant with persistent suppression of key mediators of anabolic responses. We provide novel insights into the effects of the timing of exercise-induced overload and recovery on signal transduction pathways and transcriptional activity regulating skeletal muscle mass in vivo.

AB - PURPOSE: Regulation of skeletal muscle mass is highly dependent on contractile loading. The purpose of this study was to examine changes in growth factor and inflammatory pathways following high-frequency resistance training. METHODS: Using a novel design in which male Sprague-Dawley rats undertook a "stacked" resistance training protocol designed to generate a summation of transient exercise-induced signaling responses (four bouts of three sets × 10 repetitions of squat exercise, separated by 3 h of recovery), we determined the effects of high training frequency on signaling pathways and transcriptional activity regulating muscle mass. RESULTS: The stacked training regimen resulted in acute suppression of insulin-like growth factor 1 mRNA abundance (P < 0.05) and Akt phosphorylation (P < 0.05), an effect that persisted 48 h after the final training bout. Conversely, stacked training elicited a coordinated increase in the expression of tumor necrosis factor alpha, inhibitor kappa B kinase alpha/beta activity (P < 0.05), and p38 mitogen-activated protein kinase phosphorylation (P < 0.05) at 3 h after each training bout. In addition, the stacked series of resistance exercise bouts induced an increase in p70 S6 kinase phosphorylation 3 h after bouts ×3 and ×4, independent of the phosphorylation state of Akt. CONCLUSIONS: Our results indicate that high resistance training frequency extends the transient activation of inflammatory signaling cascades, concomitant with persistent suppression of key mediators of anabolic responses. We provide novel insights into the effects of the timing of exercise-induced overload and recovery on signal transduction pathways and transcriptional activity regulating skeletal muscle mass in vivo.

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DO - 10.1249/mss.0b013e31815729b6

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JO - Medicine and science in sports

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