Early time course of akt phosphorylation after endurance and resistance exercise

Donny M. Camera, Johann Edge, Michael J. Short, John A. Hawley, Vernon G. Coffey

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Abstract

Purpose: The aim of this study was to determine the early time course of exercise-induced signaling after divergent contractile activity associated with resistance and endurance exercise. Methods: Sixteen male subjects were randomly assigned to either a cycling (CYC; n = 8, 60 min, 70% V⊙O2peak) or resistance (REX; n = 8, 8×5 leg extension, 80% one-repetition maximum, 3-min recovery) exercise group. Serial muscle biopsies were obtained from vastus lateralis at rest before, immediately after, and after 15, 30, and 60 min of passive recovery to determine early signaling responses after exercise. Results: There were comparable increases from rest in AktThr308/Ser473 and mTORSer2448 phosphorylation during the postexercise time course that peaked 30-60 min after both CYC and REX (P<0.05). There were also similar patterns in p70S6K Thr389 and 4E-BP1Thr37/46 phosphorylation, but a greater magnitude of effect was observed for REX and CYC, respectively (P<0.05). However, AMPKThr172 phosphorylation was only significantly elevated after CYC (P<0.05), and we observed divergent responses for glycogen synthaseSer641 and AS160 phosphorylation that were enhanced after CYC but not REX (P<0.05). Conclusions: We show a similar time course for Akt-mTOR-S6K phosphorylation during the initial 60-min recovery period after divergent contractile stimuli. Conversely, enhanced phosphorylation status of proteins that promote glucose transport and glycogen synthesis only occurred after endurance exercise. Our results indicate that endurance and resistance exercise initiate translational signaling, but high-load, low-repetition contractile activity failed to promote phosphorylation of pathways regulating glucose metabolism.

Original languageEnglish
Pages (from-to)1843-1852
Number of pages10
JournalMedicine and Science in Sports and Exercise
Volume42
Issue number10
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

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Phosphorylation
Glycogen
70-kDa Ribosomal Protein S6 Kinases
Facilitative Glucose Transport Proteins
Quadriceps Muscle
Leg
Biopsy
Glucose
Muscles

Cite this

Camera, Donny M. ; Edge, Johann ; Short, Michael J. ; Hawley, John A. ; Coffey, Vernon G. / Early time course of akt phosphorylation after endurance and resistance exercise. In: Medicine and Science in Sports and Exercise. 2010 ; Vol. 42, No. 10. pp. 1843-1852.
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abstract = "Purpose: The aim of this study was to determine the early time course of exercise-induced signaling after divergent contractile activity associated with resistance and endurance exercise. Methods: Sixteen male subjects were randomly assigned to either a cycling (CYC; n = 8, 60 min, 70{\%} V⊙O2peak) or resistance (REX; n = 8, 8×5 leg extension, 80{\%} one-repetition maximum, 3-min recovery) exercise group. Serial muscle biopsies were obtained from vastus lateralis at rest before, immediately after, and after 15, 30, and 60 min of passive recovery to determine early signaling responses after exercise. Results: There were comparable increases from rest in AktThr308/Ser473 and mTORSer2448 phosphorylation during the postexercise time course that peaked 30-60 min after both CYC and REX (P<0.05). There were also similar patterns in p70S6K Thr389 and 4E-BP1Thr37/46 phosphorylation, but a greater magnitude of effect was observed for REX and CYC, respectively (P<0.05). However, AMPKThr172 phosphorylation was only significantly elevated after CYC (P<0.05), and we observed divergent responses for glycogen synthaseSer641 and AS160 phosphorylation that were enhanced after CYC but not REX (P<0.05). Conclusions: We show a similar time course for Akt-mTOR-S6K phosphorylation during the initial 60-min recovery period after divergent contractile stimuli. Conversely, enhanced phosphorylation status of proteins that promote glucose transport and glycogen synthesis only occurred after endurance exercise. Our results indicate that endurance and resistance exercise initiate translational signaling, but high-load, low-repetition contractile activity failed to promote phosphorylation of pathways regulating glucose metabolism.",
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Early time course of akt phosphorylation after endurance and resistance exercise. / Camera, Donny M.; Edge, Johann; Short, Michael J.; Hawley, John A.; Coffey, Vernon G.

In: Medicine and Science in Sports and Exercise, Vol. 42, No. 10, 10.2010, p. 1843-1852.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Camera, Donny M.

AU - Edge, Johann

AU - Short, Michael J.

AU - Hawley, John A.

AU - Coffey, Vernon G.

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