Dose-dependent neuroprotective effect of caffeine on a rotenone-induced rat model of parkinsonism: A histological study

Amira M. Soliman, Ahmed M. Fathalla, Ahmed A. Moustafa*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)


Several lines of evidence have demonstrated an inverse relationship between caffeine utilization and Parkinson's disease (PD) progression. Caffeine is a methylxanthine known as a non-specific inhibitor of adenosine (A2A and A1) receptors in the cerebrum and demonstrated to be a neuroprotective medication. In this study, the neuroprotective efficacy of two different doses of caffeine ranging above the usual consumption dose and below the toxic dose was investigated using histopathological and immunohistochemical methods. Thirty-two male rats were randomly divided into 4 groups, with 8 in each group: vehicle control (1 ml/kg/48 h for 12 days), rotenone (1.5 mg/kg/48 h, s.c. for 12 days), low-dose Caffeine-treated: (10 mg/kg IP. daily for 12 days), high-dose Caffeine-treated (20 mg IP daily for 12 days). Twenty-four hours after the last rotenone injection, animals were sacrificed and brains were sectioned and prepared for histopathological staining with hematoxylinand eosin, cresyl violet and Mallory's phosphotungestic acid haematoxylinand for immunohistochemical staining of tyrosine hydroxylase. Our study showed that the treatment with caffeine improved histopathological degeneration in the substantia nigra parts compacta (SNpc) neurons and hindered the reduction in dopamine concentration caused by rotenone. We also found that a higher dose of caffeine was more effective against histopathological degeneration. These results suggest that caffeine has a dose-dependent neuroprotective effect.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalNeuroscience Letters
Publication statusPublished - 3 Jun 2016
Externally publishedYes


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