Distinct in vitro myelopoiesis is dependent on the self-renewal of hematopoietic progenitors

R A Hinton, P Papathanasiou, H C O'Neill

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic-like antigen-presenting cell type (L-DC). These progenitors are also maintained in both long-term spleen cultures and co-cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L-DC progenitors by testing their capacity to colonize STX3 and produce L-DC. E14.5 FL from wild-type C57BL/6J mice was found to colonize STX3 and produce L-DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short-term production of low numbers of L-DC between 7 and 14 days of co-culture. The transient and weak production of L-DC by FL from Plastic E14.5 mice maps to the loss of self-renewal capacity amongst HSC. L-DC progenitors are, therefore, closely aligned with a subset of self-renewing HSC/HPC in FL.

Original languageEnglish
Pages (from-to)168-75
Number of pages8
JournalScandinavian Journal of Immunology
Volume75
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

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Myelopoiesis
Liver
Spleen
Hematopoietic Stem Cells
Coculture Techniques
Plastics
Antigen-Presenting Cells
Mesenchymal Stromal Cells
Inbred C57BL Mouse
Bone Marrow
In Vitro Techniques
Cell Line

Cite this

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title = "Distinct in vitro myelopoiesis is dependent on the self-renewal of hematopoietic progenitors",
abstract = "Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic-like antigen-presenting cell type (L-DC). These progenitors are also maintained in both long-term spleen cultures and co-cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L-DC progenitors by testing their capacity to colonize STX3 and produce L-DC. E14.5 FL from wild-type C57BL/6J mice was found to colonize STX3 and produce L-DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short-term production of low numbers of L-DC between 7 and 14 days of co-culture. The transient and weak production of L-DC by FL from Plastic E14.5 mice maps to the loss of self-renewal capacity amongst HSC. L-DC progenitors are, therefore, closely aligned with a subset of self-renewing HSC/HPC in FL.",
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Distinct in vitro myelopoiesis is dependent on the self-renewal of hematopoietic progenitors. / Hinton, R A; Papathanasiou, P; O'Neill, H C.

In: Scandinavian Journal of Immunology, Vol. 75, No. 2, 02.2012, p. 168-75.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Distinct in vitro myelopoiesis is dependent on the self-renewal of hematopoietic progenitors

AU - Hinton, R A

AU - Papathanasiou, P

AU - O'Neill, H C

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AB - Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic-like antigen-presenting cell type (L-DC). These progenitors are also maintained in both long-term spleen cultures and co-cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L-DC progenitors by testing their capacity to colonize STX3 and produce L-DC. E14.5 FL from wild-type C57BL/6J mice was found to colonize STX3 and produce L-DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short-term production of low numbers of L-DC between 7 and 14 days of co-culture. The transient and weak production of L-DC by FL from Plastic E14.5 mice maps to the loss of self-renewal capacity amongst HSC. L-DC progenitors are, therefore, closely aligned with a subset of self-renewing HSC/HPC in FL.

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