Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells

Thomas J. Peters, Andrea Albieri, Estela Bevilacqua, Belinda M. Chapman, Linda H. Crane, Gary P. Hamlin, Motoharu Seiki, Michael J. Soares

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the Rcho-1 trophoblast culture system as a model for studying trophoblast invasion and to examine stage-specific expression of enzyme(s) potentially participating in rat trophoblast giant cell invasive behavior. The invasive behavior of the differentiating Rcho-1 trophoblast cells was demonstrated using Matrigel invasion chambers. Gelatin zymography and Western blot analysis of conditioned medium from differentiating Rcho-1 trophoblast cell cultures and rat ectoplacental cone outgrowths revealed a differentiation-dependent increase in gelatinase B/matrix metalloproteinase (MMP-9). Nothern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of Rcho-1 trophoblast or ectoplacental cone cells also showed increasing expression of MMP-9 accompanying cell differentiation. Rcho-1 trophoblast cells stably transfected with MMP-9 promoter/luciferase reporter constructs exhibited a differentiation-dependent increase in MMP-9 promoter activation. In conclusion, trophoblast giant cell differentiation is characterized by transcriptional activation of the MMP-9 gene and appearance of the invasive phenotype.

Original languageEnglish
Pages (from-to)287-296
Number of pages10
JournalCell and Tissue Research
Volume295
Issue number2
DOIs
Publication statusPublished - 2 Feb 1999
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 9
Trophoblasts
Matrix Metalloproteinases
Cones
Rats
Giant Cells
Chemical activation
Cell Differentiation
Polymerase chain reaction
RNA-Directed DNA Polymerase
Gelatin
Conditioned Culture Medium
Luciferases
Cell culture
Genes
Reverse Transcriptase Polymerase Chain Reaction
Transcriptional Activation
Cell Culture Techniques
Western Blotting
Enzymes

Cite this

Peters, Thomas J. ; Albieri, Andrea ; Bevilacqua, Estela ; Chapman, Belinda M. ; Crane, Linda H. ; Hamlin, Gary P. ; Seiki, Motoharu ; Soares, Michael J. / Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells. In: Cell and Tissue Research. 1999 ; Vol. 295, No. 2. pp. 287-296.
@article{cccc3047b3c447ccbea803544a669197,
title = "Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells",
abstract = "The purpose of this study was to evaluate the Rcho-1 trophoblast culture system as a model for studying trophoblast invasion and to examine stage-specific expression of enzyme(s) potentially participating in rat trophoblast giant cell invasive behavior. The invasive behavior of the differentiating Rcho-1 trophoblast cells was demonstrated using Matrigel invasion chambers. Gelatin zymography and Western blot analysis of conditioned medium from differentiating Rcho-1 trophoblast cell cultures and rat ectoplacental cone outgrowths revealed a differentiation-dependent increase in gelatinase B/matrix metalloproteinase (MMP-9). Nothern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of Rcho-1 trophoblast or ectoplacental cone cells also showed increasing expression of MMP-9 accompanying cell differentiation. Rcho-1 trophoblast cells stably transfected with MMP-9 promoter/luciferase reporter constructs exhibited a differentiation-dependent increase in MMP-9 promoter activation. In conclusion, trophoblast giant cell differentiation is characterized by transcriptional activation of the MMP-9 gene and appearance of the invasive phenotype.",
author = "Peters, {Thomas J.} and Andrea Albieri and Estela Bevilacqua and Chapman, {Belinda M.} and Crane, {Linda H.} and Hamlin, {Gary P.} and Motoharu Seiki and Soares, {Michael J.}",
year = "1999",
month = "2",
day = "2",
doi = "10.1007/s004410051235",
language = "English",
volume = "295",
pages = "287--296",
journal = "Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)",
issn = "0302-766X",
publisher = "Springer",
number = "2",

}

Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells. / Peters, Thomas J.; Albieri, Andrea; Bevilacqua, Estela; Chapman, Belinda M.; Crane, Linda H.; Hamlin, Gary P.; Seiki, Motoharu; Soares, Michael J.

In: Cell and Tissue Research, Vol. 295, No. 2, 02.02.1999, p. 287-296.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Differentiation-dependent expression of gelatinase B/matrix metalloproteinase-9 in trophoblast cells

AU - Peters, Thomas J.

AU - Albieri, Andrea

AU - Bevilacqua, Estela

AU - Chapman, Belinda M.

AU - Crane, Linda H.

AU - Hamlin, Gary P.

AU - Seiki, Motoharu

AU - Soares, Michael J.

PY - 1999/2/2

Y1 - 1999/2/2

N2 - The purpose of this study was to evaluate the Rcho-1 trophoblast culture system as a model for studying trophoblast invasion and to examine stage-specific expression of enzyme(s) potentially participating in rat trophoblast giant cell invasive behavior. The invasive behavior of the differentiating Rcho-1 trophoblast cells was demonstrated using Matrigel invasion chambers. Gelatin zymography and Western blot analysis of conditioned medium from differentiating Rcho-1 trophoblast cell cultures and rat ectoplacental cone outgrowths revealed a differentiation-dependent increase in gelatinase B/matrix metalloproteinase (MMP-9). Nothern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of Rcho-1 trophoblast or ectoplacental cone cells also showed increasing expression of MMP-9 accompanying cell differentiation. Rcho-1 trophoblast cells stably transfected with MMP-9 promoter/luciferase reporter constructs exhibited a differentiation-dependent increase in MMP-9 promoter activation. In conclusion, trophoblast giant cell differentiation is characterized by transcriptional activation of the MMP-9 gene and appearance of the invasive phenotype.

AB - The purpose of this study was to evaluate the Rcho-1 trophoblast culture system as a model for studying trophoblast invasion and to examine stage-specific expression of enzyme(s) potentially participating in rat trophoblast giant cell invasive behavior. The invasive behavior of the differentiating Rcho-1 trophoblast cells was demonstrated using Matrigel invasion chambers. Gelatin zymography and Western blot analysis of conditioned medium from differentiating Rcho-1 trophoblast cell cultures and rat ectoplacental cone outgrowths revealed a differentiation-dependent increase in gelatinase B/matrix metalloproteinase (MMP-9). Nothern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of Rcho-1 trophoblast or ectoplacental cone cells also showed increasing expression of MMP-9 accompanying cell differentiation. Rcho-1 trophoblast cells stably transfected with MMP-9 promoter/luciferase reporter constructs exhibited a differentiation-dependent increase in MMP-9 promoter activation. In conclusion, trophoblast giant cell differentiation is characterized by transcriptional activation of the MMP-9 gene and appearance of the invasive phenotype.

UR - http://www.scopus.com/inward/record.url?scp=0033006021&partnerID=8YFLogxK

U2 - 10.1007/s004410051235

DO - 10.1007/s004410051235

M3 - Article

VL - 295

SP - 287

EP - 296

JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)

JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)

SN - 0302-766X

IS - 2

ER -