Different effects of raised [K+](o) on membrane potential and contraction in mouse fast- and slow-twitch muscle

Simeon P. Cairns, Wayne A. Hing, John R. Slack, Roland G. Mills, Denis S. Loiselle

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Abstract

Increasing extracellular K+ concentration ([K+](o)) from 4 to 7-14 mM reduced both tetanic force and resting membrane potential (E(m)) in isolated slow-twitch soleus and fast-twitch extensor digitorum longus (EDL) muscles of the mouse. The tetanic force-[K+](o) relation-ships showed a greater force loss over 8-11 mM [K+](o) in soleus than EDL, mainly because the E(m) was 2- 3 mV less negative at each [K+](o) in soleus. The tetanic force-resting E(m) relationships show that force was reduced in two phases: phase 1 (E(m) < -60 mV), a 20% force decline in which the relationships superimposed in soleus and EDL, and phase 2 (E(m) -60 to -55 mV), a marked force decline that was steeper in EDL than soleus. Additionally in phase 2, longer stimulation pulses restored tetanic force; the twitch force-stimulation strength relationship was shifted toward higher voltages; caffeine, a myoplasmic Ca2+ concentration elevator, increased maximum force; and twitch force fell abruptly. We suggest that 1) the K+-depressed force is due to reduced Ca2+ release resulting from an altered action potential profile (phase 1) and inexcitable fibers due to an increased action potential threshold (phase 2), and 2) K+ contributes to fatigue in both fast- and slow-twitch muscle when it causes depolarization to about -60 mV.

Original languageEnglish
Pages (from-to)C598-C611
Number of pages14
JournalAmerican Journal of Physiology - Cell Physiology
Volume273
Issue number2
DOIs
Publication statusPublished - 1 Oct 1997
Externally publishedYes

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Membrane Potentials
Action Potentials
Elevators and Escalators
Muscles
Ships
Caffeine
Fatigue

Cite this

Cairns, Simeon P. ; Hing, Wayne A. ; Slack, John R. ; Mills, Roland G. ; Loiselle, Denis S. / Different effects of raised [K+](o) on membrane potential and contraction in mouse fast- and slow-twitch muscle. In: American Journal of Physiology - Cell Physiology. 1997 ; Vol. 273, No. 2 . pp. C598-C611.
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Different effects of raised [K+](o) on membrane potential and contraction in mouse fast- and slow-twitch muscle. / Cairns, Simeon P.; Hing, Wayne A.; Slack, John R.; Mills, Roland G.; Loiselle, Denis S.

In: American Journal of Physiology - Cell Physiology, Vol. 273, No. 2 , 01.10.1997, p. C598-C611.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Different effects of raised [K+](o) on membrane potential and contraction in mouse fast- and slow-twitch muscle

AU - Cairns, Simeon P.

AU - Hing, Wayne A.

AU - Slack, John R.

AU - Mills, Roland G.

AU - Loiselle, Denis S.

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