Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection

Muriel Soden, Helen Vasudevan, Brenda Roberts, Robert Coelen, Gary Hamlin, Subash Vasudevan, Justin La Brooy*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

68 Citations (Scopus)

Abstract

Objective. To document the histology of Ross River virus (RRV) arthritis and to examine inflamed synovium for vital RNA. Methods. Biopsy tissue from the inflamed knees of 12 patients with RRV infection was studied using conventional and immunostaining techniques. Reverse transcriptase-polymerase chain reaction technology was used to probe for the presence of vital RNA in the synovial biopsy samples and in serum. Results. Hyperplasia of the synovial lining layer, vascular proliferation, and mononuclear cell infiltration were the main histologic changes. RRV RNA was found in knee biopsy tissue that was obtained from 2 patients at 5 weeks after the onset of symptoms. Conclusion. RRV RNA was identified in inflamed synovium more than a month after symptoms began. Inflammation was apparent in the absence of detectable virus in the majority of patients.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalArthritis and Rheumatism
Volume43
Issue number2
DOIs
Publication statusPublished - 1 Feb 2000
Externally publishedYes

Fingerprint

Ross River virus
Synovial Membrane
Virus Diseases
RNA
Biopsy
Knee
Reverse Transcriptase Polymerase Chain Reaction
Arthritis
Hyperplasia
Blood Vessels
Histology
Cell Proliferation
Viruses
Inflammation
Technology
Serum

Cite this

Soden, Muriel ; Vasudevan, Helen ; Roberts, Brenda ; Coelen, Robert ; Hamlin, Gary ; Vasudevan, Subash ; La Brooy, Justin. / Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection. In: Arthritis and Rheumatism. 2000 ; Vol. 43, No. 2. pp. 365-369.
@article{bd9ba774fd3247f599e14fcf48af7d84,
title = "Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection",
abstract = "Objective. To document the histology of Ross River virus (RRV) arthritis and to examine inflamed synovium for vital RNA. Methods. Biopsy tissue from the inflamed knees of 12 patients with RRV infection was studied using conventional and immunostaining techniques. Reverse transcriptase-polymerase chain reaction technology was used to probe for the presence of vital RNA in the synovial biopsy samples and in serum. Results. Hyperplasia of the synovial lining layer, vascular proliferation, and mononuclear cell infiltration were the main histologic changes. RRV RNA was found in knee biopsy tissue that was obtained from 2 patients at 5 weeks after the onset of symptoms. Conclusion. RRV RNA was identified in inflamed synovium more than a month after symptoms began. Inflammation was apparent in the absence of detectable virus in the majority of patients.",
author = "Muriel Soden and Helen Vasudevan and Brenda Roberts and Robert Coelen and Gary Hamlin and Subash Vasudevan and {La Brooy}, Justin",
year = "2000",
month = "2",
day = "1",
doi = "10.1002/1529-0131(200002)43:2<365::AID-ANR16>3.0.CO;2-E",
language = "English",
volume = "43",
pages = "365--369",
journal = "Arthritis Care and Research",
issn = "0004-3591",
publisher = "John Wiley & Sons",
number = "2",

}

Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection. / Soden, Muriel; Vasudevan, Helen; Roberts, Brenda; Coelen, Robert; Hamlin, Gary; Vasudevan, Subash; La Brooy, Justin.

In: Arthritis and Rheumatism, Vol. 43, No. 2, 01.02.2000, p. 365-369.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection

AU - Soden, Muriel

AU - Vasudevan, Helen

AU - Roberts, Brenda

AU - Coelen, Robert

AU - Hamlin, Gary

AU - Vasudevan, Subash

AU - La Brooy, Justin

PY - 2000/2/1

Y1 - 2000/2/1

N2 - Objective. To document the histology of Ross River virus (RRV) arthritis and to examine inflamed synovium for vital RNA. Methods. Biopsy tissue from the inflamed knees of 12 patients with RRV infection was studied using conventional and immunostaining techniques. Reverse transcriptase-polymerase chain reaction technology was used to probe for the presence of vital RNA in the synovial biopsy samples and in serum. Results. Hyperplasia of the synovial lining layer, vascular proliferation, and mononuclear cell infiltration were the main histologic changes. RRV RNA was found in knee biopsy tissue that was obtained from 2 patients at 5 weeks after the onset of symptoms. Conclusion. RRV RNA was identified in inflamed synovium more than a month after symptoms began. Inflammation was apparent in the absence of detectable virus in the majority of patients.

AB - Objective. To document the histology of Ross River virus (RRV) arthritis and to examine inflamed synovium for vital RNA. Methods. Biopsy tissue from the inflamed knees of 12 patients with RRV infection was studied using conventional and immunostaining techniques. Reverse transcriptase-polymerase chain reaction technology was used to probe for the presence of vital RNA in the synovial biopsy samples and in serum. Results. Hyperplasia of the synovial lining layer, vascular proliferation, and mononuclear cell infiltration were the main histologic changes. RRV RNA was found in knee biopsy tissue that was obtained from 2 patients at 5 weeks after the onset of symptoms. Conclusion. RRV RNA was identified in inflamed synovium more than a month after symptoms began. Inflammation was apparent in the absence of detectable virus in the majority of patients.

UR - http://www.scopus.com/inward/record.url?scp=0034084175&partnerID=8YFLogxK

U2 - 10.1002/1529-0131(200002)43:2<365::AID-ANR16>3.0.CO;2-E

DO - 10.1002/1529-0131(200002)43:2<365::AID-ANR16>3.0.CO;2-E

M3 - Article

VL - 43

SP - 365

EP - 369

JO - Arthritis Care and Research

JF - Arthritis Care and Research

SN - 0004-3591

IS - 2

ER -