Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.

Denver C Surrao, Stuart Skabo, Yu-Qian Chau, Jason Ionnis Limnios, Kinnari J. Shelat, Qin Liu

Research output: Contribution to conferenceAbstractResearchpeer-review

Abstract

Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch’s membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).
Original languageEnglish
DOIs
Publication statusPublished - 30 Mar 2016
EventThe 10th World Biomaterials Congress - Montreal, Canada
Duration: 17 May 201622 May 2016
Conference number: 10th
http://www.wbc2016.org/

Conference

ConferenceThe 10th World Biomaterials Congress
Abbreviated titleWBC 2016
CountryCanada
CityMontreal
Period17/05/1622/05/16
Internet address

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Bruch Membrane
Macular Degeneration
Tissue Engineering
Membranes
Polyglactin 910
Biomimetics
Porosity
Electric Impedance
Polymers
Collagen
Epithelium
Immunohistochemistry
Cell Membrane
Polymerase Chain Reaction
In Vitro Techniques
poly(lactide)
Therapeutics

Cite this

Surrao, D. C., Skabo, S., Chau, Y-Q., Limnios, J. I., Shelat, K. J., & Liu, Q. (2016). Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.. Abstract from The 10th World Biomaterials Congress, Montreal, Canada. https://doi.org/10.3389/conf.FBIOE.2016.01.01616
Surrao, Denver C ; Skabo, Stuart ; Chau, Yu-Qian ; Limnios, Jason Ionnis ; Shelat, Kinnari J. ; Liu, Qin. / Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering. Abstract from The 10th World Biomaterials Congress, Montreal, Canada.
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abstract = "Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch’s membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40{\%}, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).",
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Surrao, DC, Skabo, S, Chau, Y-Q, Limnios, JI, Shelat, KJ & Liu, Q 2016, 'Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.' The 10th World Biomaterials Congress, Montreal, Canada, 17/05/16 - 22/05/16, . https://doi.org/10.3389/conf.FBIOE.2016.01.01616

Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering. / Surrao, Denver C; Skabo, Stuart; Chau, Yu-Qian; Limnios, Jason Ionnis; Shelat, Kinnari J.; Liu, Qin.

2016. Abstract from The 10th World Biomaterials Congress, Montreal, Canada.

Research output: Contribution to conferenceAbstractResearchpeer-review

TY - CONF

T1 - Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.

AU - Surrao, Denver C

AU - Skabo, Stuart

AU - Chau, Yu-Qian

AU - Limnios, Jason Ionnis

AU - Shelat, Kinnari J.

AU - Liu, Qin

PY - 2016/3/30

Y1 - 2016/3/30

N2 - Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch’s membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).

AB - Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch’s membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).

UR - https://www.frontiersin.org/10.3389/conf.FBIOE.2016.01.01616/event_abstract

UR - http://tech4pco.com/wbc2016/flipbook/WBC2016.pdf

UR - https://www.frontiersin.org/events/10th_World_Biomaterials_Congress/2893

U2 - 10.3389/conf.FBIOE.2016.01.01616

DO - 10.3389/conf.FBIOE.2016.01.01616

M3 - Abstract

ER -

Surrao DC, Skabo S, Chau Y-Q, Limnios JI, Shelat KJ, Liu Q. Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.. 2016. Abstract from The 10th World Biomaterials Congress, Montreal, Canada. https://doi.org/10.3389/conf.FBIOE.2016.01.01616