Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males

Daniel R. Moore, Jose Areta, Vernon G. Coffey, Trent Stellingwerff, Stuart M. Phillips, Louise M. Burke, Marilyn Cléroux, Jean Philippe Godin, John A. Hawley

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Abstract

Background: The pattern of protein intake following exercise may impact whole-body protein turnover and net protein retention. We determined the effects of different protein feeding strategies on protein metabolism in resistance-trained young men. Methods. Participants were randomly assigned to ingest either 80g of whey protein as 8x10g every 1.5h (PULSE; n=8), 4x20g every 3h (intermediate, INT; n=7), or 2x40g every 6h (BOLUS; n=8) after an acute bout of bilateral knee extension exercise (4x10 repetitions at 80% maximal strength). Whole-body protein turnover (Q), synthesis (S), breakdown (B), and net balance (NB) were measured throughout 12h of recovery by a bolus ingestion of [ 15N]glycine with urinary [15N]ammonia enrichment as the collected end-product. Results: PULSE Q rates were greater than BOLUS (∼19%, P<0.05) with a trend towards being greater than INT (∼9%, P=0.08). Rates of S were 32% and 19% greater and rates of B were 51% and 57% greater for PULSE as compared to INT and BOLUS, respectively (P<0.05), with no difference between INT and BOLUS. There were no statistical differences in NB between groups (P=0.23); however, magnitude-based inferential statistics revealed likely small (mean effect90%CI; 0.590.87) and moderate (0.800.91) increases in NB for PULSE and INT compared to BOLUS and possible small increase (0.421.00) for INT vs. PULSE. Conclusion: We conclude that the pattern of ingested protein, and not only the total daily amount, can impact whole-body protein metabolism. Individuals aiming to maximize NB would likely benefit from repeated ingestion of moderate amounts of protein (∼20g) at regular intervals (∼3h) throughout the day.

Original languageEnglish
Article number91
JournalNutrition and Metabolism
Volume9
DOIs
Publication statusPublished - 2012
Externally publishedYes

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Exercise
Proteins
Eating
Ammonia
Glycine
iodonitrotetrazolium
Knee

Cite this

Moore, Daniel R. ; Areta, Jose ; Coffey, Vernon G. ; Stellingwerff, Trent ; Phillips, Stuart M. ; Burke, Louise M. ; Cléroux, Marilyn ; Godin, Jean Philippe ; Hawley, John A. / Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males. In: Nutrition and Metabolism. 2012 ; Vol. 9.
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title = "Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males",
abstract = "Background: The pattern of protein intake following exercise may impact whole-body protein turnover and net protein retention. We determined the effects of different protein feeding strategies on protein metabolism in resistance-trained young men. Methods. Participants were randomly assigned to ingest either 80g of whey protein as 8x10g every 1.5h (PULSE; n=8), 4x20g every 3h (intermediate, INT; n=7), or 2x40g every 6h (BOLUS; n=8) after an acute bout of bilateral knee extension exercise (4x10 repetitions at 80{\%} maximal strength). Whole-body protein turnover (Q), synthesis (S), breakdown (B), and net balance (NB) were measured throughout 12h of recovery by a bolus ingestion of [ 15N]glycine with urinary [15N]ammonia enrichment as the collected end-product. Results: PULSE Q rates were greater than BOLUS (∼19{\%}, P<0.05) with a trend towards being greater than INT (∼9{\%}, P=0.08). Rates of S were 32{\%} and 19{\%} greater and rates of B were 51{\%} and 57{\%} greater for PULSE as compared to INT and BOLUS, respectively (P<0.05), with no difference between INT and BOLUS. There were no statistical differences in NB between groups (P=0.23); however, magnitude-based inferential statistics revealed likely small (mean effect90{\%}CI; 0.590.87) and moderate (0.800.91) increases in NB for PULSE and INT compared to BOLUS and possible small increase (0.421.00) for INT vs. PULSE. Conclusion: We conclude that the pattern of ingested protein, and not only the total daily amount, can impact whole-body protein metabolism. Individuals aiming to maximize NB would likely benefit from repeated ingestion of moderate amounts of protein (∼20g) at regular intervals (∼3h) throughout the day.",
author = "Moore, {Daniel R.} and Jose Areta and Coffey, {Vernon G.} and Trent Stellingwerff and Phillips, {Stuart M.} and Burke, {Louise M.} and Marilyn Cl{\'e}roux and Godin, {Jean Philippe} and Hawley, {John A.}",
year = "2012",
doi = "10.1186/1743-7075-9-91",
language = "English",
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Moore, DR, Areta, J, Coffey, VG, Stellingwerff, T, Phillips, SM, Burke, LM, Cléroux, M, Godin, JP & Hawley, JA 2012, 'Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males', Nutrition and Metabolism, vol. 9, 91. https://doi.org/10.1186/1743-7075-9-91

Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males. / Moore, Daniel R.; Areta, Jose; Coffey, Vernon G.; Stellingwerff, Trent; Phillips, Stuart M.; Burke, Louise M.; Cléroux, Marilyn; Godin, Jean Philippe; Hawley, John A.

In: Nutrition and Metabolism, Vol. 9, 91, 2012.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males

AU - Moore, Daniel R.

AU - Areta, Jose

AU - Coffey, Vernon G.

AU - Stellingwerff, Trent

AU - Phillips, Stuart M.

AU - Burke, Louise M.

AU - Cléroux, Marilyn

AU - Godin, Jean Philippe

AU - Hawley, John A.

PY - 2012

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N2 - Background: The pattern of protein intake following exercise may impact whole-body protein turnover and net protein retention. We determined the effects of different protein feeding strategies on protein metabolism in resistance-trained young men. Methods. Participants were randomly assigned to ingest either 80g of whey protein as 8x10g every 1.5h (PULSE; n=8), 4x20g every 3h (intermediate, INT; n=7), or 2x40g every 6h (BOLUS; n=8) after an acute bout of bilateral knee extension exercise (4x10 repetitions at 80% maximal strength). Whole-body protein turnover (Q), synthesis (S), breakdown (B), and net balance (NB) were measured throughout 12h of recovery by a bolus ingestion of [ 15N]glycine with urinary [15N]ammonia enrichment as the collected end-product. Results: PULSE Q rates were greater than BOLUS (∼19%, P<0.05) with a trend towards being greater than INT (∼9%, P=0.08). Rates of S were 32% and 19% greater and rates of B were 51% and 57% greater for PULSE as compared to INT and BOLUS, respectively (P<0.05), with no difference between INT and BOLUS. There were no statistical differences in NB between groups (P=0.23); however, magnitude-based inferential statistics revealed likely small (mean effect90%CI; 0.590.87) and moderate (0.800.91) increases in NB for PULSE and INT compared to BOLUS and possible small increase (0.421.00) for INT vs. PULSE. Conclusion: We conclude that the pattern of ingested protein, and not only the total daily amount, can impact whole-body protein metabolism. Individuals aiming to maximize NB would likely benefit from repeated ingestion of moderate amounts of protein (∼20g) at regular intervals (∼3h) throughout the day.

AB - Background: The pattern of protein intake following exercise may impact whole-body protein turnover and net protein retention. We determined the effects of different protein feeding strategies on protein metabolism in resistance-trained young men. Methods. Participants were randomly assigned to ingest either 80g of whey protein as 8x10g every 1.5h (PULSE; n=8), 4x20g every 3h (intermediate, INT; n=7), or 2x40g every 6h (BOLUS; n=8) after an acute bout of bilateral knee extension exercise (4x10 repetitions at 80% maximal strength). Whole-body protein turnover (Q), synthesis (S), breakdown (B), and net balance (NB) were measured throughout 12h of recovery by a bolus ingestion of [ 15N]glycine with urinary [15N]ammonia enrichment as the collected end-product. Results: PULSE Q rates were greater than BOLUS (∼19%, P<0.05) with a trend towards being greater than INT (∼9%, P=0.08). Rates of S were 32% and 19% greater and rates of B were 51% and 57% greater for PULSE as compared to INT and BOLUS, respectively (P<0.05), with no difference between INT and BOLUS. There were no statistical differences in NB between groups (P=0.23); however, magnitude-based inferential statistics revealed likely small (mean effect90%CI; 0.590.87) and moderate (0.800.91) increases in NB for PULSE and INT compared to BOLUS and possible small increase (0.421.00) for INT vs. PULSE. Conclusion: We conclude that the pattern of ingested protein, and not only the total daily amount, can impact whole-body protein metabolism. Individuals aiming to maximize NB would likely benefit from repeated ingestion of moderate amounts of protein (∼20g) at regular intervals (∼3h) throughout the day.

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