Cultivation of corneal endothelial cells from sheep

Najla Khaled Al Abdulsalam, Nigel L. Barnett, Damien G. Harkin, Jennifer Walshe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Research is currently under way to produce tissue engineered corneal endothelium transplants for therapeutic use in humans. This work requires the use of model animals, both for the supply of corneal endothelial cells (CECs) for experimentation, and to serve as recipients for test transplants. A variety of species can be used, however, a number of important advantages can be gained by using sheep as transplant recipients. The purpose of the present study was therefore to develop a method for culturing sheep CECs that would be suitable for the eventual construction of corneal endothelium grafts destined for sheep subjects. A method was established for culturing sheep CECs and these were compared to cultured human CECs. Results showed that cultured sheep and human CECs had similar growth characteristics when expanded from corneal endothelium explants on gelatin-coated plates, and achieved similar cell densities after several weeks. Furthermore, the markers zonula occludens-1, N-cadherin and sodium potassium ATPase could be immunodetected in similar staining patterns at cell boundaries of cultured CECs from both species. This work represents the first detailed study of sheep CEC cultures, and is the first demonstration of their similarities to human CEC cultures. Our results indicate that sheep CECs would be an appropriate substitute for human CECs when developing methods to produce tissue engineered corneal endothelium transplants.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalExperimental Eye Research
Volume173
DOIs
Publication statusPublished - 1 Aug 2018
Externally publishedYes

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Sheep
Endothelial Cells
Corneal Endothelium
Transplants
Cell Culture Techniques
Sodium-Potassium-Exchanging ATPase
Tight Junctions
Therapeutic Uses
Cadherins
Gelatin
Cultured Cells
Animal Models
Cell Count
Staining and Labeling
Growth
Research

Cite this

Al Abdulsalam, Najla Khaled ; Barnett, Nigel L. ; Harkin, Damien G. ; Walshe, Jennifer. / Cultivation of corneal endothelial cells from sheep. In: Experimental Eye Research. 2018 ; Vol. 173. pp. 24-31.
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Cultivation of corneal endothelial cells from sheep. / Al Abdulsalam, Najla Khaled; Barnett, Nigel L.; Harkin, Damien G.; Walshe, Jennifer.

In: Experimental Eye Research, Vol. 173, 01.08.2018, p. 24-31.

Research output: Contribution to journalArticleResearchpeer-review

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