Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

Vernon G. Coffey, Henriette Pilegaard, Andrew P. Garnham, Brendan J. O'Brien, John A. Hawley

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Abstract

We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 ± 6.3 yr, body mass of 73.2 ± 4.5 kg, peak O2 uptake (V̇O2peak) of 54.0 ± 5.7 ml·kg-1·min-1] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% V̇O2peak) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1Ser473 were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling (∼55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise (∼52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling (∼45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1α mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."

Original languageEnglish
Pages (from-to)1187-1197
Number of pages11
JournalJournal of Applied Physiology
Volume106
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Fingerprint

Skeletal Muscle
Exercise
Messenger RNA
Phosphorylation
Ribosomal Protein S6 Kinases
Muscles
Hexokinase
Quadriceps Muscle
Insulin-Like Growth Factor I
Fingers
Leg
Biopsy

Cite this

Coffey, Vernon G. ; Pilegaard, Henriette ; Garnham, Andrew P. ; O'Brien, Brendan J. ; Hawley, John A. / Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. In: Journal of Applied Physiology. 2009 ; Vol. 106, No. 4. pp. 1187-1197.
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Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. / Coffey, Vernon G.; Pilegaard, Henriette; Garnham, Andrew P.; O'Brien, Brendan J.; Hawley, John A.

In: Journal of Applied Physiology, Vol. 106, No. 4, 04.2009, p. 1187-1197.

Research output: Contribution to journalArticleResearchpeer-review

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