Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth

Nicolas Fossat; Tania Radziewic; Vanessa Jones; Karin Tourle; Patrick P.L. Tam

doi:10.1002/dvg.22920

Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth

Nicolas Fossat^*, Tania Radziewic, Vanessa Jones, Karin Tourle, Patrick P.L. Tam

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Rbm47 encodes a RNA binding protein that is necessary for Cytidine to Uridine RNA editing. Rbm47^gt/gt mutant mice that harbor inactivated Rbm47 display poor viability. Here it was determined that the loss of Rbm47^gt/gt offspring is due to embryonic lethality at mid-gestation. It was further showed that growth of the surviving Rbm47^gt/gt mutants is impaired. Rbm47 is expressed in both the visceral endoderm and the definitive endoderm. Using the utility of the switchable FlEx gene-trap cassette and the activity of Cre and FLP recombinases to generate mice that conditionally inactivate and restore Rbm47 function in tissue-specific manner, it was demonstrated that Rbm47 function is required in the embryo proper, and not the visceral endoderm, for viability and growth.

Original language	English
Pages (from-to)	115-122
Number of pages	8
Journal	Genesis
Volume	54
Issue number	3
DOIs	https://doi.org/10.1002/dvg.22920
Publication status	Published - 1 Mar 2016
Externally published	Yes

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Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growthAccepted author manuscript, 1.17 MBLicence: Other

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title = "Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth",

abstract = "Rbm47 encodes a RNA binding protein that is necessary for Cytidine to Uridine RNA editing. Rbm47gt/gt mutant mice that harbor inactivated Rbm47 display poor viability. Here it was determined that the loss of Rbm47gt/gt offspring is due to embryonic lethality at mid-gestation. It was further showed that growth of the surviving Rbm47gt/gt mutants is impaired. Rbm47 is expressed in both the visceral endoderm and the definitive endoderm. Using the utility of the switchable FlEx gene-trap cassette and the activity of Cre and FLP recombinases to generate mice that conditionally inactivate and restore Rbm47 function in tissue-specific manner, it was demonstrated that Rbm47 function is required in the embryo proper, and not the visceral endoderm, for viability and growth.",

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T1 - Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth

AU - Fossat, Nicolas

AU - Radziewic, Tania

AU - Jones, Vanessa

AU - Tourle, Karin

AU - Tam, Patrick P.L.

PY - 2016/3/1

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AB - Rbm47 encodes a RNA binding protein that is necessary for Cytidine to Uridine RNA editing. Rbm47gt/gt mutant mice that harbor inactivated Rbm47 display poor viability. Here it was determined that the loss of Rbm47gt/gt offspring is due to embryonic lethality at mid-gestation. It was further showed that growth of the surviving Rbm47gt/gt mutants is impaired. Rbm47 is expressed in both the visceral endoderm and the definitive endoderm. Using the utility of the switchable FlEx gene-trap cassette and the activity of Cre and FLP recombinases to generate mice that conditionally inactivate and restore Rbm47 function in tissue-specific manner, it was demonstrated that Rbm47 function is required in the embryo proper, and not the visceral endoderm, for viability and growth.

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Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth

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