Conditional restoration and inactivation of Rbm47 reveal its tissue-context requirement for viability and growth

Nicolas Fossat*, Tania Radziewic, Vanessa Jones, Karin Tourle, Patrick P.L. Tam

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Rbm47 encodes a RNA binding protein that is necessary for Cytidine to Uridine RNA editing. Rbm47gt/gt mutant mice that harbor inactivated Rbm47 display poor viability. Here it was determined that the loss of Rbm47gt/gt offspring is due to embryonic lethality at mid-gestation. It was further showed that growth of the surviving Rbm47gt/gt mutants is impaired. Rbm47 is expressed in both the visceral endoderm and the definitive endoderm. Using the utility of the switchable FlEx gene-trap cassette and the activity of Cre and FLP recombinases to generate mice that conditionally inactivate and restore Rbm47 function in tissue-specific manner, it was demonstrated that Rbm47 function is required in the embryo proper, and not the visceral endoderm, for viability and growth.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalGenesis
Volume54
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

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