TY - JOUR
T1 - Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk
AU - Khalesi, Mohammad
AU - Waterhouse, Mary
AU - Whiteman, David C.
AU - Johns, Richard
AU - Rosendahl, Cliff
AU - Hackett, Timothy
AU - Pollak, Thomas
AU - Kimlin, Michael G.
AU - Hacker, Elke
AU - Neale, Rachel E.
N1 - Funding Information:
This study was completely funded by the National Health and Medical Research Council (NHMRC) Centre for Research Excellence in Sun and Health (CRESH) established within Queensland University of Technology (QUT) in Brisbane, Australia. In addition, MK has been funded by CRESH, QUT Health Top-Up, and QIMR Berghofer Medical Research Institute Top-Up Scholarships. DCW holds an NHMRC Principal Research Fellowship. REN holds an NHMRC Senior Research Fellowship.
Publisher Copyright:
© 2016 The International Society of Dermatology
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Background: There is some evidence that basal cell carcinomas (BCCs) arising on different anatomic sites and developing to different histological subtypes differ in their pathophysiology. The expression of a number of proteins, including PTCH1, COX-2, p53, and Ki-67, is frequently altered in BCC development. Objectives: This study sought to determine whether protein expression differs between BCCs at different anatomic sites and of different histological subtypes. Methods: Expression of PTCH1, COX-2, p53, and Ki-67 proteins was compared between: (i) BCCs arising on the head (n = 55) and trunk (n = 53), and (ii) nodular (n = 52) and superficial (n = 43) BCCs. The intensity of immunohistochemistry (IHC) staining (low, moderate, strong, very strong) for PTCH1 and COX-2 proteins was measured and the proportions of p53- and Ki-67-positive cells quantified. Results: The proportion of cells expressing Ki-67 was higher in tumor tissue than in non-malignant epidermis, whereas the opposite was found for PTCH1. The IHC staining intensity for PTCH1 was substantially greater in truncal BCCs than in BCCs on the head (odds ratio [OR] 3.82, 95% confidence interval [CI] 1.63–8.96). The intensity of staining for PTCH1 was greater for superficial than for nodular BCCs (OR 3.70, 95% CI 1.53–8.97), and superficial BCCs showed a higher proportion of Ki-67-positive cells (OR 5.57, 95% CI 1.66–18.67). Conclusions: These differences suggest that the pathophysiology of BCC differs between lesions on the head and trunk and between nodular and superficial subtypes, perhaps indicating differences in their etiology.
AB - Background: There is some evidence that basal cell carcinomas (BCCs) arising on different anatomic sites and developing to different histological subtypes differ in their pathophysiology. The expression of a number of proteins, including PTCH1, COX-2, p53, and Ki-67, is frequently altered in BCC development. Objectives: This study sought to determine whether protein expression differs between BCCs at different anatomic sites and of different histological subtypes. Methods: Expression of PTCH1, COX-2, p53, and Ki-67 proteins was compared between: (i) BCCs arising on the head (n = 55) and trunk (n = 53), and (ii) nodular (n = 52) and superficial (n = 43) BCCs. The intensity of immunohistochemistry (IHC) staining (low, moderate, strong, very strong) for PTCH1 and COX-2 proteins was measured and the proportions of p53- and Ki-67-positive cells quantified. Results: The proportion of cells expressing Ki-67 was higher in tumor tissue than in non-malignant epidermis, whereas the opposite was found for PTCH1. The IHC staining intensity for PTCH1 was substantially greater in truncal BCCs than in BCCs on the head (odds ratio [OR] 3.82, 95% confidence interval [CI] 1.63–8.96). The intensity of staining for PTCH1 was greater for superficial than for nodular BCCs (OR 3.70, 95% CI 1.53–8.97), and superficial BCCs showed a higher proportion of Ki-67-positive cells (OR 5.57, 95% CI 1.66–18.67). Conclusions: These differences suggest that the pathophysiology of BCC differs between lesions on the head and trunk and between nodular and superficial subtypes, perhaps indicating differences in their etiology.
UR - http://www.scopus.com/inward/record.url?scp=84987791184&partnerID=8YFLogxK
U2 - 10.1111/ijd.13276
DO - 10.1111/ijd.13276
M3 - Article
C2 - 27126210
AN - SCOPUS:84987791184
SN - 0011-9059
VL - 55
SP - 1096
EP - 1105
JO - International Journal of Dermatology
JF - International Journal of Dermatology
IS - 10
ER -